5-Azacytidine and sodium butyrate induce expression of aromatase in fibroblasts from chickens carrying the henny feathering trait but not from wild-type chickens.
5-Azacytidine and sodium butyrate induce expression of aromatase in fibroblasts from chickens carrying the henny feathering trait but not from wild-type chickens.
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5-氮杂胞苷和丁酸钠诱导具有母鸡羽毛性状的鸡的成纤维细胞表达芳香酶，但野生型鸡则不然。
DOI：
10.1073/pnas.82.9.3005
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发表时间：
1985
影响因子：
11.1
通讯作者：
Leshin,M
中科院分区：
文献类型：
--
作者：
Leshin,M
具有母鸡羽毛特征的雄性鸡具有雌性羽毛模式。在两种有母鸡羽毛的品种中，Sebright bantam和Golden Campine，由于芳香化酶（一种将雄激素转化为雌激素的细胞色素P-450酶）的活性增加，雌激素的合成增加。该酶的活性在组织切片和培养的杂合和纯合鸟的成纤维细胞中均升高。相比之下，对照鸡腺外组织的芳香酶活性非常低，从这些组织培养的成纤维细胞中检测不到芳香酶活性。目前的研究表明，两种已知的改变基因表达的药物——5-氮杂胞苷和丁酸钠——显著诱导Sebright和Campine成纤维细胞的芳香酶活性表达，但对野生型鸡成纤维细胞的芳香酶活性没有影响。芳香化酶的诱导是特异性的，因为鸡成纤维细胞中的另外两种微粒体酶——一种是芳香化酶复合物的组成部分，另一种是与芳香化酶不同的细胞色素P-450氧化酶——不受这些药物的显著影响。对这一独特突变的进一步研究将有助于深入了解基因在分化过程中切换到不可诱导状态的机制。
Male chickens with the henny feathering trait have a female feathering pattern. In two henny-feathered breeds, the Sebright bantam and the Golden Campine, the synthesis of estrogen is increased as a consequence of increased activity of aromatase, a cytochrome P-450 enzyme that converts androgen to estrogen. The activity of the enzyme is elevated in tissue slices and in cultured fibroblasts from heterozygous and homozygous birds of both breeds. In contrast, aromatase activity is very low in extraglandular tissues from control chickens and is undetectable in fibroblasts cultured from these tissues. The current studies show that two agents known to alter gene expression--5-azacytidine and sodium butyrate--markedly induce expression of aromatase activity in Sebright and Campine fibroblasts but have no effect on aromatase activity in fibroblasts from wild-type chickens. Induction of aromatase is specific since two other microsomal enzymes in chicken fibroblasts--one, a component of the aromatase enzyme complex and the other a cytochrome P-450 oxidase distinct from the aromatase--are not significantly affected by these agents. Further study of this unique mutation should provide insight into the mechanisms by which genes are switched to an uninducible state during differentiation.