Phosphoproteins are components of mitotic microtubule organizing centers.
Phosphoproteins are components of mitotic microtubule organizing centers.
复制标题
磷蛋白是有丝分裂微管组织中心的组成部分。
DOI：
10.1073/pnas.81.14.4439
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Borisy,GG
中科院分区：
文献类型：
--
作者：
Vandre,DD;Davis,FM;Rao,PN;Borisy,GG
蛋白质磷酸化被认为是有丝分裂起始和完成的重要调控机制。使用单克隆抗体识别一类磷蛋白丰富的有丝分裂细胞，我们证明了本地化的一个子集，这些磷蛋白的几个离散的有丝分裂结构。斑片状免疫荧光存在于间期核，但核免疫荧光显着增加是明显的前期。随后的有丝分裂阶段表明，免疫反应物质是特别明显的微管组织中心，即，中心体，动粒，和中体。强烈的中心体定位发生在前中期-前中期的过渡，并持续到改造的核膜在早期G1。有丝分裂细胞的细胞质中也含有免疫反应性物质，与没有细胞质荧光染色的间期细胞形成鲜明对比。在固定之前，通过用0.15%Triton X-100对细胞进行短暂裂解来去除大部分弥散免疫荧光细胞质物质。洗涤剂溶解后，有丝分裂微管组织中心的剩余的免疫反应性材料的本地化表明，它们含有重要的磷蛋白结构成分，也许，在有丝分裂相-间期过渡。
Protein phosphorylation has been suggested as an important control mechanism for the events leading toward the initiation and completion of mitosis. Using a monoclonal antibody recognizing a class of phosphoproteins abundant in mitotic cells, we demonstrated the localization of a subset of these phosphoproteins to several discrete mitotic structures. Patchy immunofluorescence was present in the interphase nuclei, but a significant increase in nuclear immunofluorescence was apparent at prophase. Subsequent mitotic stages demonstrated that immunoreactive material was particularly apparent at microtubule organizing centers, namely, centrosomes, kinetochores, and midbodies. Intense centrosomal localization occurred at the prophase-prometaphase transition and persisted until the reformation of the nuclear membrane in early G1. The cytoplasm of mitotic cells also contained immunoreactive material in sharp contrast to interphase cells that exhibited no cytoplasmic fluorescent staining. Much of the diffuse immunofluorescent cytoplasmic material was removed by a brief lysis of the cells with 0.15% Triton X-100 prior to fixation. The localization of the remaining immunoreactive material after detergent lysis to mitotic microtubule organizing centers suggests that they contain phosphoprotein structural components important, perhaps, in the mitotic phase-interphase transition.