Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus
Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus
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DOI：
10.1128/jcm.43.4.1982-1984.2005
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发表时间：
2005-04-01
影响因子：
9.4
通讯作者：
Montassier, HJ
中科院分区：
文献类型：
--
作者：
Gibertoni, AM;Montassier, MDS;Montassier, HJ
采用重组酵母表达N蛋白酶联免疫吸附试验（Y-N-ELISA），以酵母表达的传染性支气管炎病毒（IBV） M41株核衣壳(N)多肽为抗原。Y-N-ELISA检测鸡IBV血清抗体快速、灵敏、特异，优于市售ELISA。
A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.