In vivo studies of Gallibacterium anatis infection in chickens
In vivo studies of Gallibacterium anatis infection in chickens
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DOI：
10.1080/03079450310001652059
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发表时间：
2004-04-01
期刊：
影响因子：
2.8
通讯作者：
Bisgaard, M
中科院分区：
文献类型：
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作者：
Bojesen, AM;Nielsen, OL;Bisgaard, M
本研究的目的是观察正常或免疫抑制的鸡静脉或腹腔注射一种特征良好的鸭芽孢杆菌菌株后的病理变化。研究人员使用了两组30只15周龄的商业褐蛋鸡，这些鸡都经过了筛选，并发现镓杆菌微生物呈阴性。一组给予5-氟尿嘧啶促进异嗜性粒细胞耗竭，另一组给予生理盐水治疗。10 d后，每组15只鸡静脉或腹腔接种3.3 × 10(7)个猪螺旋体菌落形成单位的12656-12菌株。在感染后3、12或24小时处死鸡亚群，检查是否有病变。用培养法和荧光原位杂交法检测肝脏和脾脏。静脉注射感染的鸟类在正常和免疫抑制的鸟类中均表现出严重的败血症病变。只有后者有死亡率记录，总体死亡率为73%。免疫状态正常的腹腔感染鸡在接种部位出现不同程度的局限性化脓性腹膜炎，而免疫抑制的鸡则倾向于累及整个腹膜和腹部器官。这与先前对自然感染的描述相似，可能代表了一种有用的感染模型，用于详细分析镓杆菌的毒力因子和发病机制。
The aim of the present study was to investigate the pathology in normal or immunosuppressed chickens followed intravenous or intraperitoneal inoculation with a well-characterized strain of Gallibacterium anatis. Two groups of 30 15-week-old commercial brown laying chickens were used, having been screened and found negative for Gallibacterium organisms. One group was treated with 5-fluorouracil to promote heterophil depletion, while the other was saline treated. Ten days later 15 chickens from each group were inoculated either intravenously or intraperitoneally with 3.3 X 10(7) colony-forming units of G. anatis strain 12656-12. Subsets of chickens were sacrificed at 3, 12 or 24 h post-infection and examined for lesions. Livers and spleens were examined by culture and by fluorescent in situ hybridization. Intravenously infected birds showed severe septicaemic lesions in both the normal and immunosuppressed birds. Mortality was recorded only in the latter, with an overall rate of 73%. The intraperitoneally infected chickens of normal immune status showed various degrees of localized purulent peritonitis at the inoculation site, but in the immunosuppressed birds the entire peritoneum tended to be involved along with the abdominal organs. This was similar to previous descriptions of natural infections and may represent a useful infection model for detailed analysis of Gallibacterium virulence factors and pathogenesis.