GATA-type transcriptional factor Gat1 regulates nitrogen uptake and polymalic acid biosynthesis in polyextremotolerant fungus Aureobasidium pullulans
GATA-type transcriptional factor Gat1 regulates nitrogen uptake and polymalic acid biosynthesis in polyextremotolerant fungus Aureobasidium pullulans
复制标题
GATA™型转录因子Gat1调节多极耐菌出芽短梗霉的氮吸收和聚苹果酸生物合成
DOI：
10.1111/1462-2920.14841
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发表时间：
2020
影响因子：
5.1
通讯作者：
Zou Xiang
中科院分区：
文献类型：
--
作者：
Song Xiaodan;Wang Yongkang;Wang Pan;Pu Guihong;Zou Xiang
聚苹果酸（PMA）是一种由多极端耐受性真菌普鲁兰毛霉（aubasasidium pululans）产生的新型生物聚合物。在这项研究中，研究了一个调控氮吸收和PMA生物合成的GATA家族转录因子Gat1。在基因过表达的突变体中，PMA的产量增加到11.2%，但当基因从a的基因组中敲除gengat1时，PMA的产量下降到49.1%。支链淀粉。野生型和突变型菌株（∆gat1和OE::gat1）的转录组对比分析显示，共有23个差异表达基因与氧化磷酸化、核糖体生物发生和氮代谢有关。在氮限制胁迫下，无论首选氮（谷氨酰胺，Gln）还是非首选氮（脯氨酸，Pro），细胞中70%的Gat1位于核细胞质中，导致氮吸收增加和PMA生物合成调节。定量RT‐PCR结果显示，糖酵解途径中的葡萄糖激酶（GLK）和glyoxylate分流途径中的苹果酸合成酶（MLS）可能受到Gat1和氮浓度（Gln或Pro）的交叉调控，因此，突变菌株（OE:: Gat1）中GLK过表达，导致PMA滴度和产量分别提高12.6%和13.0%。这些结果表明，Gat1可能在PMA生物合成过程中对氮和碳代谢的双重调控中发挥重要作用。
Polymalic acid (PMA) is a novel biopolymer produced by the polyextremotolerant fungusAureobasidium pullulans. In this study, a GATA‐family transcriptional factor, Gat1, which regulates nitrogen uptake and PMA biosynthesis, was investigated. PMA production increased to 11.2% in the mutant overexpressinggat1but decreased to 49.1% of the PMA titre whengat1was knocked out from the genome ofA. pullulans. Comparative transcriptome analysis of wild‐type and mutant strains (∆gat1and OE::gat1) revealed that 23 common differentially expressed genes were related to oxidative phosphorylation, ribosome biogenesis, and nitrogen metabolism. Under nitrogen‐limited stress, regardless of the preferred nitrogen (glutamine, Gln) or non‐preferred nitrogen (proline, Pro), 70% of Gat1 in the cells was located in the nucleus–cytoplasm, which resulted in an increase in nitrogen uptake and PMA biosynthesis regulation. Quantitative RT‐PCR revealed that glucosekinase (GLK) in the glycolytic pathway and malate synthase (MLS) in the glyoxylate shunt pathway may be cross‐regulated by Gat1 and nitrogen concentration (Gln or Pro), Therefore,glkwas overexpressed in mutant strain (OE::gat1), which resulted in an increased PMA titre and yield of 12.6% and 13.0% respectively. These findings indicate that Gat1 may play an important role in the dual regulation of the nitrogen and carbon metabolisms in PMA biosynthesis.