ADHERENCE AND INGESTION OF ESCHERICHIA-COLI SEROTYPE-055 BY TROPHOZOITES OF ENTAMOEBA-HISTOLYTICA
ADHERENCE AND INGESTION OF ESCHERICHIA-COLI SEROTYPE-055 BY TROPHOZOITES OF ENTAMOEBA-HISTOLYTICA
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DOI：
10.1128/iai.40.3.882-887.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
MIRELMAN, D
中科院分区：
文献类型：
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作者：
BRACHA, R;MIRELMAN, D
溶组织埃希氏菌细胞表面存在的碳水化合物结合活性介导了两种细菌的黏附，即大肠杆菌O55型和沙门氏菌绿色素O50。低分子碳水化合物(10 mg/ml)，如半乳糖、乳糖和N-乙酰半乳糖胺，以及积雪草素和从E.coliO55中提取的脂多糖可抑制黏附。细菌轻微的高碘酸氧化抑制了它们的粘附性；热灭活、戊二醛固定或伽马射线照射没有影响。用戊二醛、细胞松弛素B或COLD(5度)对滋养体进行预处理。C)废除遵守。这些处理都不影响带有甘露糖结合能力的I型菌毛的附着。这些发现进一步支持了早期对阿米巴如何与细菌相互作用的观察[即细菌对阿米巴毒力的增强]。
Carbohydrate-binding activity present on the E. histolytica cell surfaces mediated the adherence of 2 types of bacteria, E. coli serotype O55 and Salmonella greenside O50. Adherence was inhibited by low-MW carbohydrates (10 mg/ml) such as galactose, lactose and N-acetylgalactosamine, and by asialofetuin and the lipopolysaccharide extracted from E. coli O55. Mild periodate oxidation of the bacteria inhibited their adherence; heat inactivation, glutaraldehyde fixation or .gamma.-irradiation had no effect. Pretreatment of trophozoites with glutaraldehyde, cytochalasin B or cold (5.degree. C) abolished adherence. None of these treatments affected the attachment of bacteria bearing type I pili with mannose-binding capacity. These findings lend further support to earlier observations on how amoebae interact with bacteria [i.e., bacterial enhancement of amoebal virulence].