Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis
Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis
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分析 CCN2 在肾小管上皮细胞中的功能，重点关注肾纤维形成
DOI：
10.1007/978-1-0716-2744-0_28
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Okada Hirokazu
中科院分区：
文献类型：
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作者：
Amano Hiroaki;Inoue Tsutomu;Kusano Takeru;Okada Hirokazu
肾间质纤维化是所有肾脏疾病发展过程中的最终共同途径，并最终导致慢性肾脏疾病。CCN 2是肾间质纤维化发病机制中的重要因素，分析其功能可以导致慢性肾脏疾病的治疗。由于CCN 2敲除小鼠在发育上是致死的，因此条件性敲除小鼠的产生对于体内分析是必不可少的。由于CCN 2在肾脏中的多种细胞中表达，包括足细胞、系膜细胞、周细胞和肾小管上皮细胞，因此有必要对在纤维化中起核心作用的细胞进行细胞特异性验证。然而，使用Cre/loxP系统在体内的细胞特异性验证仅在系膜细胞中进行。在我们的研究计划中，我们专注于CCN 2在肾小管上皮细胞中的作用。在这份报告中，我们介绍了建立一个肾小管上皮细胞特异性基因敲除模型及其分析方法。
Renal interstitial fibrosis is the final common pathway in the process of all kidney diseases, and it results in chronic kidney disease. CCN2 is an important factor in the pathogenesis of renal interstitial fibrosis, and analysis of its function can lead to treatments for chronic kidney disease. Since CCN2 knockout mice are developmentally lethal, generation of conditional knockout mice is essential for in vivo analysis. Since CCN2 is expressed in a variety of cells in the kidney, including podocytes, mesangial cells, pericytes, and tubular epithelial cells, it is necessary to perform cell-specific verification of the cells that play a central role in fibrosis. However, cell-specific validation using the Cre/loxP system in vivo has only been performed in mesangial cells. In our research program, we are focusing on the role of CCN2 in tubular epithelial cells in renal fibrogenesis. In this report, we introduce the creation of a tubular epithelial cell-specific knockout model and method of its analysis.