The effects of beta-bungarotoxin on the morphogenesis of taste papillae and taste buds in the mouse.
The effects of beta-bungarotoxin on the morphogenesis of taste papillae and taste buds in the mouse.
复制标题
β-银环蛇毒素对小鼠味觉乳头和味蕾形态发生的影响。
DOI：
10.1093/chemse/24.1.7
复制
发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Du,Y
中科院分区：
文献类型：
--
作者：
Morris-Wiman,J;Basco,E;Du,Y
虽然长期以来人们认为味觉神经的神经支配对于味蕾的维持是必不可少的，但尚不清楚神经支配在味觉乳头和味蕾发育的形态发生中起什么作用（如果有的话）。进行以下研究以确定感觉神经支配的缺乏对小鼠中味觉乳头的发育和味蕾的形成具有什么影响。将妊娠第12天（gd12）的定时妊娠雌性小鼠（n = 3）麻醉，并将1 µ l（1 µ g/µ l）β-银环蛇毒素（β-BTX）溶液（一种破坏感觉和运动神经元发育的神经毒素）注射到每只母鼠两个胚胎的羊膜腔内。用PBS注射两个假手术。在出生前1天（gd18）收获胎仔，并将4个β-BTX注射胚胎、2个假胚胎和2个对照胚胎固定在缓冲多聚甲醛中。连续切片检查味觉乳头和味蕾的存在和形态。在注射β-BTX的舌中未观察到神经轮廓。 虽然在β-BTX舌上存在轮廓状乳头，但只能识别出5个菌状乳头。在假手术和对照胎仔中，大部分菌状乳头轮廓（24%）和轮廓乳头上存在味蕾;相比之下，β-BTX胎仔中没有味蕾与味觉乳头相关。这些结果暗示了神经支配在味觉乳头和味蕾形态发生中的重要作用。
Although it has been long accepted that innervation by a taste nerve is essential for maintenance of taste buds, it is not clear what role, if any, innervation plays in the morphogenesis of taste papillae and taste bud development. The following study was undertaken to determine what effects lack of sensory innervation have on the development of taste papillae and the formation of taste buds in the mouse. Timed-pregnant female mice (n= 3) at gestational day 12 (gd12) were anesthetized and a 1 µl solution (1 µg/µl) of β -bungarotoxin (β-BTX), a neurotoxin that disrupts sensory and motor neuron development, was injected into the amniotic cavity of two embryos per dam. Two shams were injected with PBS. Fetuses were harvested at gd18, 1 day before birth, and four β -BTX-injected embryos, two shams and two controls were fixed in buffered paraformaldehyde. Serial sections were examined for the presence and morphology of taste papillae and taste buds. No nerve profiles were observed in β-BTX-injected tongues. Although circumvallate papillae were present on β-BTX tongues, only five fungiform papillae could be identified. Taste buds were present on a large percentage of fungiform papillae profiles (24% and on circumvallate papillae in sham and control fetuses; in contrast, no taste buds were associated with taste papillae in β-BTX fetuses. These results implicate a significant role for innervation in taste papillae and taste bud morphogenesis.