DOMINANT T-CELL RECEPTOR BETA-CHAIN GENE REARRANGEMENTS INDICATE CLONAL EXPANSION IN THE RHEUMATOID JOINT
DOMINANT T-CELL RECEPTOR BETA-CHAIN GENE REARRANGEMENTS INDICATE CLONAL EXPANSION IN THE RHEUMATOID JOINT
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DOI：
10.1111/j.1365-3083.1990.tb02750.x
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发表时间：
1990-01-01
影响因子：
3.7
通讯作者：
BREEDVELD, FC
中科院分区：
文献类型：
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作者：
MILTENBURG, AMM;VANLAAR, JM;BREEDVELD, FC
T细胞受体（TcR）β和δ在从类风湿性关节炎（RA）患者的滑膜（SM）（n = 5）或滑液（SF）（n = 2）培养的抗CD 3扩增的T细胞群中研究基因重排。显性TcR β-在所有测试的患者中证实了C β 1的链基因重排，并且发现每个患者1-3个扩增的克隆。在一个SM样品（两个克隆）和一个SF样品（一个克隆）中检测到C β 2的克隆重排。TcR δ。基因在所有测试的样品中被删除。我们的结论是克隆优势可能会发现在扩大的T细胞群体从SM和SF RA患者。使用C β 1或C β 2基因片段可以存在多个克隆。
T-cell receptor (TcR) .beta. and .delta. gene rearrangements were studied in anti-CD3 expanded T-cell populations cultured from the synovial membrane (SM) (n = 5) or synovial fluid (SF) (n = 2) of rheumatoid arthritis (RA) patients. Dominant TcR .beta.-chain gene rearrangements to C.beta.1 were demonstrated in all the patients tested and 1-3 expanded clones per patient were found. Clonal rearrangements to C.beta.2 were detected in one SM sample (two clones) and one SF sample (one clone). The TcR .delta. gene was deleted in all the samples tested. We conclude that clonal dominance may be found in expanded T-cell populations from SM and SF of RA patients. Multiple clones may be present, either using the C.beta.1 or C.beta.2 gene segment.