Expression of Circadian Neuropeptides in the Hypothalamus of Adult Mice is Affected by Postnatal Light Experience
Expression of Circadian Neuropeptides in the Hypothalamus of Adult Mice is Affected by Postnatal Light Experience
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DOI：
10.1111/j.1365-2826.2009.01914.x
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发表时间：
2009-11-01
影响因子：
3.2
通讯作者：
Canal, M. M.
中科院分区：
文献类型：
--
作者：
Smith, L.;Canal, M. M.
下丘脑的视交叉上核（SCN）是哺乳动物的主要起搏器，控制着生理和行为的昼夜节律。发育过程中的环境光对昼夜节律行为有长期影响，但目前尚不清楚大脑中的相关适应是什么。在本研究中，我们研究了在不同光照环境下饲养的成年小鼠在哺乳期和断奶后同步光照/黑暗周期的SCN中运动活动的昼夜节律表现，以及精氨酸-血管加压素（AVP）和血管肠多肽（VIP）的表达。我们发现，与在光/暗循环或恒定黑暗下饲养的小鼠相比，在恒定光照下饲养的动物具有更高的振幅和更稳定的活动节律，同时SCN中VIP-和avp免疫染色水平较低。AVP在室旁核和视上核这两个接收SCN投射的脑区表达也存在差异。这些结果表明，出生后的光照经历可能会影响生物钟功能和生物钟输出，并暗示对激素稳态和昼夜节律行为的控制。
The suprachiasmatic nuclei (SCN) of the hypothalamus are the principal pacemaker in mammals, controlling daily, circadian rhythms in physiology and behaviour. Environmental light during development has long-term effects on circadian behaviour, but it is still unclear what the relevant adaptations within the brain are. In the present study, we examined the manifestation of the circadian rhythm of locomotor activity, and the expression of arginine-vasopressin (AVP) and vasointestinal polypeptide (VIP) in the SCN of adult mice reared under different light environments during the suckling period, and synchronised to light/dark cycles after weaning. We found that animals reared under constant light had higher amplitude and more stable activity rhythms, together with lower levels of VIP- and AVP-immunostaining in the SCN, compared to mice reared under light/dark cycles or constant darkness. Differences in AVP expression were also found in the paraventricular nucleus and the supraoptic nucleus, two brain areas which receive SCN projections. These results indicate that the postnatal light experience may affect clock function and clock output, and suggest implications for the control of hormonal homeostasis and circadian behaviour.