In ovo neuromuscular stimulation alters the skeletal muscle phenotype of the chick
In ovo neuromuscular stimulation alters the skeletal muscle phenotype of the chick
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DOI：
10.1007/s10974-005-9007-8
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发表时间：
2005-02-01
影响因子：
2.7
通讯作者：
Stickland, NC
中科院分区：
文献类型：
--
作者：
Heywood, JL;McEntee, GM;Stickland, NC
4-氨基吡啶(4-AP)是一种阻断神经元钾通道的药物，能刺激神经递质乙酰胆碱(ACh)的释放，增强其在突触间隙的利用率。研究了4-AP诱导的神经肌肉刺激对胚胎雏鸡骨骼肌形成和发育的影响。受精卵在37.5摄氏度的温度下孵化，并在孵化的第三天开窗。在胚胎第10、11、12、13天，分别用含4-AP 0.2mU的L缓冲液100mU注射于胚胎第10、11、12、13天，对照组只注射100mU的L PBS。在E14、E15和E16龄时，4-AP处理(T)胚胎的平均体重比对照(C)至少增加了10%(P<；0.05)。在E15和E16，4-AP处理组胚胎的胫骨和股骨长度显著大于对照组(P<；0.05)。在E20时，4-AP处理的动物的肌纤维数量比对照动物多36.8%。胚胎16d时，处理组胚胎的单位横截面积核数显著高于对照胚胎(P<；0.01)。在E16(P<；0.01)和E20(P<；0.05)，4-AP处理组在半腱肌横切面显示出更大百分比的氧化纤维。这些结果表明，4-AP诱导的神经肌肉刺激对骨骼肌特征、小腿骨长和全身质量有显著影响。
4-aminopyridine (4-AP) is a drug that blocks the potassium channels in neurons and stimulates the release of the neurotransmitter acetylcholine (ACh), enhancing its availability at the synaptic cleft. The effects of 4-AP induced neuromuscular stimulation on skeletal muscle formation and development were investigated in embryonic chicks. Fertile white Leghorn eggs were incubated at 37.5 degrees C and windowed on day three of incubation. On embryonic days (E) 10, 11, 12 and 13 half of the eggs were injected with 100 mu l of PBS buffer containing 0.2 mu g 4-AP and the control group was administered 100 mu l of PBS only. 4-AP treated (T) embryos showed at least a 10% increase in mean body mass relative to the controls (C) (P < 0.05) at ages E14, E15 and E16. Tibia and femur lengths in the 4-AP treated embryos were significantly greater than the controls at E15 and E16 ( P < 0.05). The 4-AP treated animals had a 36.8% greater number of myofibres than the control animals at E20. Nuclear number per cross sectional area in the M. Semitendinosus was significantly greater (P < 0.01) at E16 in the treated compared to the control embryos. The 4-AP treated group exhibited a greater percentage area of oxidative fibres in cross sections of M. Semitendinosus than the control group at E16 ( P < 0.01) and at E20 ( P < 0.05). It may be concluded from these results that 4-AP induced neuromuscular stimulation has a significant effect on skeletal muscle characteristics, leg bone length and overall body mass.