Patterning cells and their environments using multiple laminar fluid flows in capillary networks
Patterning cells and their environments using multiple laminar fluid flows in capillary networks
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DOI：
10.1073/pnas.96.10.5545
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发表时间：
1999-05-11
影响因子：
11.1
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Takayama, S;McDonald, JC;Whitesides, GM
本文描述了在毛细管系统中使用液体的层流来图案化细胞培养基质、执行图案化细胞沉积以及图案化细胞培养基。我们展示了具有不同蛋白质的细胞培养基质的图案化、彼此相邻的不同类型的细胞的图案化、化学物质向粘附细胞的图案化递送以及在选择细胞或细胞的一部分上进行酶促反应。这种方法提供了一种同时控制细胞所附着的表面特征、其附近的细胞类型以及细胞或细胞部分所暴露的介质类型的方法。该方法是实验简单，适应性强，不需要特殊的设备，除了弹性救济，可以很容易地通过快速原型制作。
This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.