In vivo aging of red cell enzymes: study of biotinylated red blood cells in rabbits.
In vivo aging of red cell enzymes: study of biotinylated red blood cells in rabbits.
复制标题
红细胞酶的体内老化：兔生物素化红细胞的研究。
DOI：
10.1002/ajh.2830330407
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发表时间：
1990
影响因子：
12.8
通讯作者：
Beutler,E
中科院分区：
文献类型：
--
作者：
Zimran,A;Forman,L;Suzuki,T;Dale,GL;Beutler,E
人们普遍认为，随着细胞的衰老，一些红细胞酶的活性会下降。然而，关于这种衰老发生的速度仍然存在争议。在本研究中，我们应用新开发的成熟网织红细胞/红细胞的特异性分离技术，以更全面地研究红细胞酶在兔体内的老化。反复采血造成贫血，用N-羟基琥珀酰亚胺生物素标记富网织红细胞，然后输注给正常兔。这些生物素化的细胞在不同的时间点通过对亲和素载体的亲和力来分离，并测定了19种红细胞酶的酶活性。我们观察到六种依赖年龄的酶--醛缩酶、谷草转氨酶、葡萄糖6-磷酸脱氢酶、己糖激酶、嘧啶5‘-核苷酸酶和丙酮酸激酶的活性呈现双相衰退。
It is generally recognized that the activities of some of the red cell enzymes decline as the cell ages. However, there is still a controversy regarding the rate at which this aging occurs. In the present study we applied newly developed technology for the specific isolation of maturing reticulocytes/erythrocytes for a more comprehensive study of in vivo aging of red cell enzymes in rabbits. Anemia was induced by repeated phlebotomy, and reticulocyte‐rich erythrocytes were labeled with N‐hydroxy succinimido‐biotin and then transfused into a normal rabbit. These biotinylated cells were isolated at various time points by their affinity for an avidin support, and the enzymatic activity of 19 red cell enzymes was measured. We observed a biphasic pattern of decay for the activity of six age‐dependent enzymes—aldolase, glutamate‐oxaloacetate transaminase, glucose 6‐phosphate dehydrogenase, hexokinase, pyrimidine 5′‐nucleotidase, and pyruvate kinase.