Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene.
Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene.
复制标题
猪孕酮受体膜成分 2 (PGRMC2) 基因的克隆、定位和分子表征
DOI：
10.1590/s1415-47572010005000057
复制
发表时间：
2010-07
影响因子：
2.1
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Chen C;Sargent C;Quilter C;Yang Z;Ren J;Affara N;Brenig B;Huang L
黄体酮通过核受体刺激经典基因组途径，并通过膜受体（例如黄体酮受体膜成分 2 (PGRMC2)）刺激非基因组途径，从而在母猪繁殖中发挥重要作用。在这项工作中，我们使用辐射杂交图谱将 PGRMC2 分配到猪的 8 号染色体上，并观察到该受体在猪中有两个转录本。大转录本的全长 cDNA 长 1858 bp，包含一个 669 bp 的开放阅读框（ORF），编码 223 个氨基酸的蛋白质。较短的转录物编码 170 个氨基酸的蛋白质。猪PGRMC2基因由三个外显子组成，长度分别为446 bp、156 bp和1259 bp。启动子序列富含 GC，缺乏典型的 TATA 框。在 208 bp 上游基因组区域中鉴定出了几个假定的顺式调控 DNA 基序。在内含子*和 3' UTR 中检测到 5 个单核苷酸多态性 (SNP)。 RT-PCR表明PGRMC2基因在所有检测的猪组织中普遍表达。
Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.