Bone marrow-derived cells are differentially involved in pathological and physiological retinal angiogenesis in mice.
Bone marrow-derived cells are differentially involved in pathological and physiological retinal angiogenesis in mice.
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DOI：
10.1016/j.bbrc.2009.12.057
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发表时间：
2010-01
影响因子：
3.1
通讯作者：
H. Zou;A. Otani;A. Oishi;Y. Yodoi;T. Kameda;H. Kojima;N. Yoshimura
中科院分区：
文献类型：
--
作者：
H. Zou;A. Otani;A. Oishi;Y. Yodoi;T. Kameda;H. Kojima;N. Yoshimura
骨髓来源的细胞已被证明在血管生成中发挥作用。虽然这些细胞已被证明可以促进血管生成，但尚不清楚这些细胞是否影响所有类型的血管生成。本研究探讨了骨髓来源的细胞在病理和生理血管生成的参与在鼠retinal.MATERIALS和METHODS的氧诱导的视网膜病变（OIR）模型被用作新生小鼠视网膜血管生成模型。为了阻断骨髓来源的细胞的影响，用来自α 137 Cs源的4-戈伊剂量的辐射照射小鼠。用放射性致密的2cm厚的铅盘在四种不同条件下进行照射：（1）H组，头部覆盖这些盘以保护眼睛免受辐射;（2）A组，全身覆盖这些盘;（3）N组，小鼠完全不屏蔽;（4）C组，将小鼠置于照射器中但不照射。在P17，显示病理和生理视网膜血管生成的视网膜区域进行了测量，并与未照射小鼠的视网膜进行比较。照射组小鼠视网膜无灌注区明显大于对照组（P<0.05），表明生理性血管生成受损。然而，簇状血管生成过程的形成在照射小鼠中更为突出（P<0.05），表明病理性血管生成是完整的。小鼠视网膜中的病理性血管生成不需要功能性骨髓源性细胞，但这些细胞对于生理性血管的形成是重要的。我们的研究结果增加了对视网膜血管生成病理学的新认识，并支持了骨髓细胞参与视网膜血管生成疾病病理或严重程度的假设。
PURPOSEBone marrow-derived cells have been shown to play roles in angiogenesis. Although these cells have been shown to promote angiogenesis, it is not yet clear whether these cells affect all types of angiogenesis. This study investigated the involvement of bone marrow-derived cells in pathological and physiological angiogenesis in the murine retina.MATERIALS AND METHODSThe oxygen-induced retinopathy (OIR) model was used as a retinal angiogenesis model in newborn mice. To block the influence of bone marrow-derived cells, the mice were irradiated with a 4-Gy dose of radiation from a137Cs source. Irradiation was performed in four different conditions with radio dense 2-cm thick lead disks; (1) H group, the head were covered with these discs to protect the eyes from radiation; (2) A group, all of the body was covered with these discs; (3) N group, mice were completely unshielded; (4) C group, mice were put in the irradiator but were not irradiated. On P17, the retinal areas showing pathological and physiological retinal angiogenesis were measured and compared to the retinas of nonirradiated mice.RESULTSAlthough irradiation induced leukocyte depletion, it did not affect the number of other cell types or body weight. Retinal nonperfusion areas were significantly larger in irradiated mice than in control mice (P<0.05), indicating that physiological angiogenesis was impaired. However, the formation of tuft-like angiogenesis processes was more prominent in the irradiated mice (P<0.05), indicating that pathological angiogenesis was intact.CONCLUSIONSBone marrow-derived cells seem to be differentially involved in the formation of physiological and pathological retinal vessels. Pathological angiogenesis in the murine retina does not require functional bone marrow-derived cells, but these cells are important for the formation of physiological vessels. Our results add a new insight into the pathology of retinal angiogenesis and bolster the hypothesis that bone marrow cells are involved in the pathology or severity of retinal angiogenic diseases.