The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis
The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis
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DOI：
10.1093/plcell/koad227
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发表时间：
2023-08-30
期刊：
影响因子：
11.6
通讯作者：
Jiang，Liwen
中科院分区：
文献类型：
--
作者：
Zhu，Ying;Zhao，Qiong;Jiang，Liwen
膜蛋白的稳态通过液泡降解和再循环的细胞途径进行微调，最终促进植物生长和细胞与环境的相互作用。内体转运所需的分选复合物（ESCRT）在调节腔内小泡（ILV）的形成和膜蛋白分选到液泡中起着重要作用。我们以前表明，植物特异性ESCRT组件FYVE结构域蛋白质所需的内体分选1（FREE 1）在植物中执行多种功能，虽然潜在的机制仍然难以捉摸。在本研究中，我们对拟南芥中的free 1-RNA突变体进行了抑制筛选，并鉴定了21个free 1（sof）突变体的抑制基因。这两个突变体sof 10和sof 641分别导致AT 5G 10370中提前终止密码子或错义突变。DRIF 1与拟南芥基因组中的一个同源基因DRIF 2具有95%的同源性。drif 1和drif 2突变体的胚胎在球形期停滞，形成扩大的多泡体（MVB），ILV数量增加。DRIF 1是一种膜相关蛋白，与逆转录组分分选连接蛋白1协调，以调节PIN-FORMED 2再循环至质膜。总之，我们的数据表明，DRIF 1是一个独特的retromer相互作用，协调FREE 1介导的ILV形成的MVBs和液泡分选膜蛋白在植物中降解。
Membrane protein homeostasis is fine-tuned by the cellular pathways for vacuolar degradation and recycling, which ultimately facilitate plant growth and cell–environment interactions. The endosomal sorting complex required for transport (ESCRT) machinery plays important roles in regulating intraluminal vesicle (ILV) formation and membrane protein sorting to vacuoles. We previously showed that the plant-specific ESCRT component FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FREE1) performs multiple functions in plants, although the underlying mechanisms remain elusive. In this study, we performed a suppressor screen of theFREE1-RNAimutant and identified and characterized 2suppressor of free1(sof) mutants in Arabidopsis (Arabidopsis thaliana). These mutants,sof10andsof641, result in a premature stop codon or a missense mutation inAT5G10370, respectively. This gene was namedDEAH and RING domain-containing protein as FREE1 suppressor 1(DRIF1).DRIF1has a homologous gene,DRIF2, in the Arabidopsis genome with 95% identity toDRIF1. The embryos ofdrif1 drif2mutants arrested at the globular stage and formed enlarged multivesicular bodies (MVBs) with an increased number of ILVs. DRIF1 is a membrane-associated protein that coordinates with retromer component sorting nexin 1 to regulate PIN-FORMED2 recycling to the plasma membrane. Altogether, our data demonstrate that DRIF1 is a unique retromer interactor that orchestrates FREE1-mediated ILV formation of MVBs and vacuolar sorting of membrane proteins for degradation in plants.