BIOLOGICAL DISTANCES ON DNA KNOTS AND LINKS: Applications to XER recombination
BIOLOGICAL DISTANCES ON DNA KNOTS AND LINKS: Applications to XER recombination
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DNA 结和链接的生物距离：XER 重组的应用
DOI：
10.1142/s0218216501000846
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发表时间：
2001
影响因子：
0.5
通讯作者：
I. Darcy
中科院分区：
文献类型：
--
作者：
I. Darcy
缠结的数学在研究重组酶时非常有用，重组酶需要DNA处于某种构型才能起作用。酶-DNA复合物的电子显微镜照片显示酶是一个带有DNA环的斑点，斑点内DNA的构型不能从电子显微镜照片中确定。然而，在某些情况下，数学可以确定酶滴内DNA的构型以及酶的作用。本文总结了分析重组酶实验的几个定理。特别是Xer重组酶，分析一种不起促发作用的酶。不幸的是，对于那些不直接起作用的酶来说，存在着无限多的可能性。提出了几个实验，以减少这个数字，并强调缠结分析的有用性和局限性。虽然没有更多的生物学假设，局部作用不能在数学上确定，但通过额外的生物学实验可以确定突触复合体的拓扑结构。
The mathematics of tangles has been very useful in studying recombinases which act processively and which require DNA to be in a certain configuration in order for the enzyme to act. Electron micrographs of the enzyme-DNA complex show the enzyme as a blob with DNA looping out of it. The configuration of the DNA within the blob cannot be determined form the electron micrographs. However, mathematics can in some cases determine the configuration of the DNA within the enzyme blob as well as the enzyme action. In this paper, several theorems used to analyze recombinase experiments are summarized. In particular Xer recombinase, an enzyme which does not act processively is analyzed. Unfortunately, for enzymes which do not act processively, infinitely many possibilities exist. Several experiments are proposed to reduce this number and to emphasize both the usefulness and limitations of tangle analysis. Although the local action cannot be mathematically determined without more biological assumptions, it is possible to determine the topology of the synaptic complex through additional biolgical experiments.