Bacterial Community Shifts in Casing Soil Before and After the Cultivation of Oudemansiella raphanipes
Bacterial Community Shifts in Casing Soil Before and After the Cultivation of Oudemansiella raphanipes
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DOI：
10.1007/s42729-022-01011-7
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发表时间：
2022-10
影响因子：
3.9
通讯作者：
W. Qin;Juan Zhao;Yu Liu;Qi Gao;Shuang Song;Shouxian Wang;Bing-bing Zhang
中科院分区：
文献类型：
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作者：
W. Qin;Juan Zhao;Yu Liu;Qi Gao;Shuang Song;Shouxian Wang;Bing-bing Zhang
覆土是萝卜根奥德蘑生产的关键步骤。覆土细菌群落对食用菌的生长发育起着重要作用。然而，知识的差距仍然是关于细菌群落的变化，在套管土壤之前和之后的培养ofO。rapanipes（CSBACO）。对栽培前、后的土壤样品进行了装箱。采用高通量16 S rRNA基因测序技术对中国竹节虫（CSBC）和中国竹节虫（CSAC）进行检测。放线菌门、变形菌门、绿弯杆菌门和酸杆菌门是O.萝卜足类占总丰度的77.32%。在培育ofO之后，细菌群落的α多样性和有益菌属Marmoricola、Nocardioides、Sphingomonas和Streptomyces的相对丰度下降，而MB-A2-108候选菌群的α多样性和相对丰度则显著增加。同时，覆土的理化性质也发生了明显的变化。土壤铵态氮和有机质含量在种植O.萝卜属。相反，在CSAC中，有效钾和pH显著增加，主要影响细菌群落。萝卜。此外，具有氨基酸和脂类代谢功能的细菌相对丰度下降，而与寄生虫和病原菌相关的细菌相对丰度增加，进一步揭示了覆土细菌群落的重要性。在CSBACO中，细菌群落的多样性、组成和潜在功能发生了显著变化。研究结果加深了对水稻土覆土细菌群落及其微生态机制的认识。萝卜。
Casing soil is a key step in the production ofOudemansiella raphanipes. Bacterial community in casing soil plays key roles in the growth and development of edible fungi. However, a knowledge gap remains regarding the bacterial community shifts in casing soil before and after the cultivation ofO. raphanipes(CSBACO). Casing soil samples before and after the cultivation ofO. raphanipes(CSBC and CSAC) were collected and examined by high-throughput 16S rRNA gene sequencing technology. Actinobacteria, Proteobacteria, Chloroflexi, and Acidobacteria were dominant phyla in casing soil ofO. raphanipes, which accounted for 77.32% of the total abundance. After the cultivation ofO. raphanipes, the alpha diversity of bacterial community and the relative abundance of some beneficial genera such asMarmoricola,Nocardioides,Sphingomonas, andStreptomycesdecreased, while that of bacteria belonging to the candidate class MB-A2-108 tolerant to adverse environment significantly increased. Meanwhile, physicochemical properties of casing soil changed significantly in CSBACO. Soil ammonium nitrogen and organic matter greatly decreased in CSAC and they mainly influenced bacterial community before the cultivation ofO. raphanipes. In contrast, available potassium and pH significantly increased in CSAC and mainly affected bacterial community after the cultivation ofO. raphanipes. Furthermore, the relative abundance of bacteria with the function of amino acid and lipid metabolism decreased, while that of bacteria functions related to parasites and pathogens increased, which further revealed the significance of bacterial community in casing soil. Diversity, composition, and the potential function of bacterial community shifted dramatically in CSBACO. The results deepen the understanding of the bacterial community and its microecological mechanism in casing soil ofO. raphanipes.