Effect of a 12-month exercise intervention on patterns of cellular proliferation in colonic crypts: A randomized controlled trial
Effect of a 12-month exercise intervention on patterns of cellular proliferation in colonic crypts: A randomized controlled trial
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DOI：
10.1158/1055-9965.epi-06-0223
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发表时间：
2006-09-01
影响因子：
3.8
通讯作者：
Lampe, Paul D.
中科院分区：
文献类型：
--
作者：
McTiernan, Anne;Yasui, Yutaka;Lampe, Paul D.
背景：结肠隐窝的结构和增殖可能是检测预防干预措施的合适生物标志物。运动诱导的结肠癌风险降低的一个假想机制可能是通过改变结肠隐窝细胞的结构和增殖。方法：通过胃肠病学实践和媒体招募在过去3年内接受结肠镜检查的健康、久坐的参与者。我们随机将100名女性和102名男性，年龄在40岁到75岁之间，分为对照组和为期12个月的中等强度有氧运动干预组，每天60分钟，每周6天，评估结肠粘膜隐窝Ki67染色细胞的数量和相对位置的变化。结果：运动者平均运动370min/wk(男性)和295min/wk(女性)(7人放弃了干预)。在男性中，Ki67阳性核的平均高度与运动量有关，较基线的变化分别为0.0%(对照组)、+0.3%(运动者250min/wk)、-1.7%(运动者250-300min/wk)和-2.4%(运动者300min/wk；P趋势=0.03)。在心肺适能(V(O2)max)增加5%的男性锻炼者中，Ki67阳性核的平均高度减少了2%，而其他锻炼者的平均高度减少了0.9%，而对照组则没有变化(P-趋势=0.05)。在其他增殖标志物中也观察到了类似的趋势。在女性中，增加运动量或增加V(O2)max并没有导致增殖标记物的显著变化。结论：12个月的中到高强度有氧运动干预导致平均运动量=250min/wk或V(O2)max增加=5%的男性结肠隐窝细胞增殖指数显著降低。
Background: Colon crypt architecture and proliferation may be appropriate biomarkers for testing prevention interventions. A hypothesized mechanism for exercise-induced colon cancer risk reduction might be through alterations in colon crypt cell architecture and proliferation.Methods: Healthy, sedentary participants with a colonoscopy within the previous 3 years were recruited through gastroenterology practices and media. We randomly assigned 100 women and 102 men, ages 40 to 75 years, to a control group or a 12-month exercise intervention of moderate-to-vigorous aerobic exercise, 60 minutes per day, 6 days per week, and assessed change in number and relative position of Ki67-stained cells in colon mucosal crypts.Results: Exercisers did a mean 370 min/wk (men) and 295 min/wk (women) of exercise (seven dropped the intervention). In men, the mean height of Ki67-positive nuclei relative to total crypt height was related to amount of exercise, with changes from baseline of 0.0% (controls), +0.3% (exercisers < 250 min/wk), -1.7% (exercisers 250-300 min/wk), and -2.4% (exercisers > 300 min/wk; P-trend = 0.03). In male exercisers whose cardiopulmonary fitness (V(O2)max) increased > 5%, the mean height of Ki67-positive nuclei decreased by 2% versus 0.9% in other exercisers, and versus no change in controls (P-trend = 0.05). Similar trends were observed in other proliferation markers. In women, increased amount of exercise or V(O2)max did not result in notable changes in proliferation markers.Conclusions: A 12-month moderate-to-vigorous intensity aerobic exercise intervention resulted in significant decreases in colon crypt cell proliferation indices in men who exercised a mean of >= 250 min/wk or whose V(O2)max increased by >= 5%.