Chlamydomonas as a tool to study tubulin polyglutamylation
Chlamydomonas as a tool to study tubulin polyglutamylation
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衣藻作为研究微管蛋白多谷氨酰化的工具
DOI：
10.1093/jmicro/dfy044
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发表时间：
2018
期刊：
影响因子：
1.8
通讯作者：
Oda Toshiyuki
中科院分区：
文献类型：
--
作者：
Kubo Tomohiro;Oda Toshiyuki
α-和β-微管蛋白的多样性由各种翻译后修饰（PTM）促进，如乙酰化、酪氨酸化、甘氨酰化、谷氨酰化、磷酸化和甲基化。这些PTM影响微管的稳定性和结构，以及微管和微管相关蛋白（包括分子马达）之间的相互作用。因此，研究微管蛋白的蛋白质末端修饰对了解细胞周期、细胞运动和细胞内运输的作用是非常重要的。微管蛋白PTM在20世纪80年代首次被研究，自那时以来已经取得了相当大的进展;可能还有其他机制尚未阐明。在这里，我们讨论了这样一个修饰，微管蛋白谷氨酰化，并介绍我们的单细胞绿色藻类莱茵衣藻真核鞭毛的研究。
The diversity of α- and β-tubulin is facilitated by various post-translational modifications (PTMs), such as acetylation, tyrosination, glycylation, glutamylation, phosphorylation and methylation. These PTMs affect the stability and structure of microtubules as well as the interaction between microtubules and microtubule-associated proteins, including molecular motors. Therefore, it is extremely important to investigate the roles of tubulin PTMs for understanding the cell cycle, cell motility and intracellular trafficking. Tubulin PTMs were first studied in the 1980s, and considerable progress has been made since then; it is likely that additional mechanisms remain yet to be elucidated. Here, we discuss one such modification, tubulin glutamylation, and introduce our research on the eukaryotic flagellum of the unicellular green algaChlamydomonas reinhardtii.