Hydrodynamic and label-free sorting of circulating tumor cells from whole blood
Hydrodynamic and label-free sorting of circulating tumor cells from whole blood
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DOI：
10.1063/1.4935563
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发表时间：
2015-11-16
影响因子：
4
通讯作者：
Franke, Thomas
中科院分区：
文献类型：
--
作者：
Geislinger, Thomas M.;Stamp, Melanie E. M.;Franke, Thomas
我们证明了不同的体外癌细胞系（MV3，MCF7和HEPG2）的连续，被动和无标记的分选，作为来自未稀释全血的循环肿瘤细胞（CTC）的模型系统，采用非惯性提升效应作为驱动力。这种纯粹粘性的排斥性细胞壁相互作用对细胞大小和变形性差异敏感，并产生高效的细胞分离和高富集因子。我们表明，该设备的性能在大范围的血细胞浓度和流速以及不同的细胞系是稳健的。收集的样品通常含有超过90%的初始注射的CTC，并且表现出超过20的平均富集因子，用于从全血样品中分选。(c)2015 AIP Publishing LLC.
We demonstrate continuous, passive, and label-free sorting of different in vitro cancer cell lines (MV3, MCF7, and HEPG2) as model systems for circulating tumor cells (CTCs) from undiluted whole blood employing the non-inertial lift effect as driving force. This purely viscous, repulsive cell-wall interaction is sensitive to cell size and deformability differences and yields highly efficient cell separation and high enrichment factors. We show that the performance of the device is robust over a large range of blood cell concentrations and flow rates as well as for the different cell lines. The collected samples usually contain more than 90% of the initially injected CTCs and exhibit average enrichment factors of more than 20 for sorting from whole blood samples. (c) 2015 AIP Publishing LLC.