Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1
Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1
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DOI：
10.1007/s11368-011-0368-x
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发表时间：
2011-04
影响因子：
3.6
通讯作者：
Chen Ma;Yue-qiang Wang;L. Zhuang;Deyin Huang;Shungui Zhou;Fangbai Li
中科院分区：
文献类型：
--
作者：
Chen Ma;Yue-qiang Wang;L. Zhuang;Deyin Huang;Shungui Zhou;Fangbai Li
PurposeThe anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) has great significance to PAHs’ natural attenuation in contaminated sites. Previous studies mainly focused on anaerobic PAH degradation by mixed cultures with nitrate, sulfate, or Fe(III) oxides as electron acceptors, and the roles of pure cultures in the process was rarely reported. The aim of this paper is to isolate a pure culture that is capable of degrading phenanthrene anaerobically with anthraquinone-2,6-disulphonate (AQDS) as the sole electron acceptor and evaluate its environmental functions.Materials and methodsA strain of pure culture was isolated from anodic solution of a microbial fuel cell via enrichment procedure with phenanthrene and AQDS under anaerobic conditions. Using the single colony isolation technique, a distinct colony was obtained and identified by the phenotypic and phylogenetic analysis. Its ability to reduce AQDS and degrade phenanthrene was conducted in serum bottles by standard anaerobic techniques (purged with 80% N2−20% CO2). The concentration of AH2QDS and fructose was quantified by UV-vis spectrophotometer at 450 and 210 nm, respectively. Cells number was determined by direct plate counting on aerobic LB agar medium. The concentration of phenanthrene was determined using HPLC.Results and discussionStrain PAH-1 was identified asPseudomonas aeruginosa. It could oxidize fructose or glucose to reduce AQDS and can support microbial growth by conserving energy from fructose to AQDS. It has the ability to degrade phenanthrene directly with AQDS as the sole electron acceptor (46.5% removal), and the microbial process may be AQDS dependent. The addition of small organic substances (fructose) could enhance the anaerobic biodegradation of phenanthrene from 46.5% to 56.7%. The anaerobic degradation of phenanthrene fits the pseudo-first-order kinetics, giving the rate constants of 0.0233/day (R2= 0.934) and 0.0328/day (R2= 0.933) for non-fructose set and fructose set, respectively.ConclusionsWe successfully isolated a facultative anaerobe,P. aeruginosastrain PAH-1. This study was the first paper reporting that a pure culture (strain PAH-1) has the ability to anaerobically degrade phenanthrene with AQDS as the sole electron acceptor. The finding also explores the environmental significance of thePseudomonasgenus.
