Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC-MS/MS
Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC-MS/MS
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DOI：
10.1530/rep-13-0313
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发表时间：
2014-03-01
期刊：
影响因子：
3.8
通讯作者：
Wang, Dong
中科院分区：
文献类型：
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作者：
Chen, Xiaoli;Zhu, Huabin;Wang, Dong
冷冻损伤是精液冷冻保存中的一个主要问题，导致影响精子功能和运动的蛋白质水平的变化。在本研究中，使用相对和绝对定量同量异位素标签（iTRAQ）标记技术结合2D LC-MS/MS分析，比较了从新鲜和冷冻解冻的猪精子中制备的蛋白质样品。共鉴定和定量了41个差异表达蛋白，其中35个蛋白在冻融精子中以较高水平表达，6个蛋白在冻融精子中以较低水平表达，平均差异至少为1.79倍（P
Cryodamage is a major problem in semen cryopreservation, causing changes in the levels of proteins that influence the function and motility of spermatozoa. In this study, protein samples prepared from fresh and frozen-thawed boar spermatozoa were compared using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique coupled to 2D LC-MS/MS analysis. A total of 41 differentially expressed proteins were identified and quantified, including 35 proteins that were present at higher levels and six proteins that were present at lower levels in frozen-thawed spermatozoa by at least a mean of 1.79-fold (P