The paradox of multiplex DNA melting on a surface.
The paradox of multiplex DNA melting on a surface.
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多重 DNA 在表面熔化的悖论。
DOI：
10.1016/j.ab.2010.09.024
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发表时间：
2011
影响因子：
2.9
通讯作者：
Benight,AlbertS
中科院分区：
文献类型：
--
作者：
Williams,Layne;Blair,Steve;Chagovetz,Alexander;Fish,DanielJ;Benight,AlbertS
在平衡条件下，目标在表面上有两种捕获方式--目标受限和探测受限。在探测器受限区域，由于低亲和力物种的反向置换机制，多重靶从表面解离的熔化曲线呈现单一的跃迁。熔化曲线不能用于解析异双链体的分析方法；只有使用单纯形体系才能得到适当的热力学。
Under equilibrium conditions, there are two regimes of target capture on a surface – target limited and probe limited. In the probe limited regime, the melting curve from multiplex target dissociation from the surface exhibits a single transition due to a reverse displacement mechanism of the low affinity species. The melting curve cannot be used in analytical methods to resolve heteroduplexes; only with the simplex system can proper thermodynamics be obtained.