Dynamic Variation in Protein-Small Molecule Interaction Observed by Double-Nanohole Optical Trapping
Dynamic Variation in Protein-Small Molecule Interaction Observed by Double-Nanohole Optical Trapping
复制标题
双纳米孔光捕获观察蛋白质-小分子相互作用的动态变化
DOI：
--
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
R. Gordon
中科院分区：
文献类型：
--
作者：
A. A. Balushi;R. Gordon
蛋白质与小分子的相互作用是其在生物体中发挥作用的基础，在药物开发中被广泛研究。在这里，我们使用金属薄膜中的双纳米孔光学陷阱来比较链霉亲和素和生物素化链霉亲和素的光学捕获动力学。在有和没有小分子结合的蛋白质之间可以看到一致和明显不同的行为。这种技术可以获得单蛋白水平的实时动态，它还具有不需要拴在表面或不需要外源标记的优点。
The interaction of proteins with small molecules is fundamental to their function in living organisms and it is widely studied in drug development. Here we compare optical trapping dynamics of streptavidin and biotinylated streptavidin using a double nanohole optical trap in a metal film. Consistent and clearly distinct behavior is seen between the protein with and without the small molecule binding. The real-time dynamics at the single protein level are accessible with this technique, which also has advantages of not requiring tethering to a surface or the need for exogeneous markers.