RESPIRATORY SYNCYTIAL VIRUS-INFECTION IN MICE
RESPIRATORY SYNCYTIAL VIRUS-INFECTION IN MICE
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DOI：
10.1128/iai.43.2.649-655.1984
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
COLLINS, AP
中科院分区：
文献类型：
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作者：
TAYLOR, G;STOTT, EJ;COLLINS, AP
人呼吸道合胞病毒A2株在BALB/c小鼠的鼻和肺中复制，病毒在老年动物中的滴度高于年轻动物。在第2 - 7天从鼻中回收病毒，在第3 - 4天达到峰值滴度，在第2 - 9天从肺中回收病毒，在第4 - 6天达到峰值滴度。感染后2 wk出现血清抗体。免疫荧光法显示肺泡细胞内有病毒抗原。组织学变化包括支气管周围和血管周围组织的单核细胞浸润，一些间质增厚和多核巨细胞形成。从接种牛呼吸道合胞病毒株的小鼠呼吸道中不能回收病毒。人呼吸道合胞病毒A2株在不同细胞系中的生长影响其对小鼠的感染性。用呼吸道合胞病毒感染BALB/c小鼠，为研究呼吸道合胞病毒的发病机制和免疫机制提供了一个重复性好的模型。
The A2 strain of human respiratory syncytial virus replicated in the nose and lung of BALB/c mice, with virus growing to higher titers in older animals than in younger animals. Virus was recovered from the nose between days 2 and 7 with peak titers on days 3 and 4, and from the lungs between day 2 and 9, with peak titers on days 4 through 6. Serum antibody developed 2 wk after infection. Viral antigen was demonstrated in the alveolar cells of the lung by immunofluorescence. Histopathological changes included infiltration by mononuclear cells of the peribronchiolar and perivascular tissue, some interstitial thickening and formation of multinucleated giant cells. Virus could not be recovered from the respiratory tract of mice inoculated with bovine strains of respiratory syncytial virus. Growth of the A2 strain of human respiratory syncytial virus in different cell lines affected its infectivity for mice. Infection of BALB/c mice with respiratory syncytial virus provides a highly reproducible model for the study of the pathogenesis of and mechanisms of immunity to this virus.