Distinct Gene Expression Profiles in Egg and Synergid Cells of Rice as Revealed by Cell Type-Specific Microarrays
Distinct Gene Expression Profiles in Egg and Synergid Cells of Rice as Revealed by Cell Type-Specific Microarrays
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DOI：
10.1104/pp.110.167502
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发表时间：
2011-02-01
期刊：
影响因子：
7.4
通讯作者：
Tsutsumi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Ohnishi, Takayuki;Takanashi, Hideki;Tsutsumi, Nobuhiro
开花植物中的双受精是指由花粉管携带的两个精细胞在释放到雌配子体的助细胞中后使卵和中央细胞都受精的过程。雌配子体细胞在受精过程中表达其独特功能的分子过程还不清楚。卵细胞和助细胞中表达的基因可能对植物生殖过程的多个阶段很重要。在这里，我们分析了全基因组基因表达的卵细胞和助细胞在水稻（水稻），模式单子叶植物，使用非酶细胞分离技术。我们发现，卵细胞和助细胞的表达谱已经指定在雌配子体的珠孔端在短的发育期间，包括三个连续的有丝分裂核分裂后大孢子生成。此外，我们还鉴定了大量在水稻卵细胞和助细胞中表达的基因，并利用Gene Ontology分析对这些基因进行了表征。分析表明，表观遗传和转录后调控机制参与这些细胞的规范和/或维护。比较水稻和拟南芥的基因表达谱发现，水稻卵/助细胞中富集的基因与拟南芥中的基因不同。
Double fertilization in flowering plants refers to a process in which two sperm cells, carried by the pollen tube, fertilize both the egg and the central cell after their release into a synergid cell of the female gametophyte. The molecular processes by which the female gametophytic cells express their unique functions during fertilization are not well understood. Genes expressed in egg and synergid cells might be important for multiple stages of the plant reproductive process. Here, we profiled genome-wide gene expression in egg and synergid cells in rice (Oryza sativa), a model monocot, using a nonenzymatic cell isolation technique. We found that the expression profiles of the egg and synergid cells were already specified at the micropylar end of the female gametophyte during the short developmental period that comprises the three consecutive mitotic nuclear divisions after megaspore generation. In addition, we identified a large number of genes expressed in the rice egg and synergid cells and characterized these genes using Gene Ontology analysis. The analysis suggested that epigenetic and posttranscriptional regulatory mechanisms are involved in the specification and/or maintenance of these cells. Comparisons between the rice profiles and reported Arabidopsis (Arabidopsis thaliana) profiles revealed that genes enriched in the egg/synergid cell of rice were distinct from those in Arabidopsis.