Elicitor hydrophobin Hyd1 interacts with Ubiquilin1-like to induce maize systemic resistance
Elicitor hydrophobin Hyd1 interacts with Ubiquilin1-like to induce maize systemic resistance
复制标题
引发子疏水蛋白 Hyd1 与 Ubiquilin1 样相互作用诱导玉米系统抗性
DOI：
10.1111/jipb.12796
复制
发表时间：
2019
影响因子：
11.4
通讯作者：
Jie Chen
中科院分区：
文献类型：
--
作者：
Chuanjin Yu;Kai Dou;Shaoqing Wang;Qiong Wu;Mi Ni;Tailong Zhang;Zhixiang Lu;Jun Tang;Jie Chen
哈茨木霉是一种对植物有益的真菌，其分泌的富含半胱氨酸的小蛋白可诱导植物防御反应;然而，参与这种诱导的分子机制在很大程度上是未知的。在这里，我们报告的II类疏水蛋白ThHyd 1作为诱导系统抗性（ISR）在植物中的激发子。免疫金标记和免疫荧光显示ThHyd 1定位于玉米根细胞质膜上。为了鉴定Hyd 1的宿主植物蛋白互作因子，我们筛选了玉米B73根cDNA文库。ThHyd 1与ubiquilin 1-like（UBL）直接相互作用。此外，UBL的N端片段主要负责与Hyd 1结合，Hyd 1的8个半胱氨酸氨基酸参与蛋白质-蛋白质相互作用。Hyd 1来自T. harzianum（Thhyd 1）和玉米ubl基因在拟南芥中共表达，协同提高植物对灰霉病的抗性。用新月弯孢菌孢子悬浮液喷雾后24 h玉米叶片中的全局基因表达的RNA测序分析表明，Thhyd 1诱导的系统抗性主要与油菜素内酯信号传导相关，可能通过BAK 1介导。茉莉酸/乙烯（JA/ET）信号也在一定程度上参与了这种反应。我们的研究结果表明，Hyd 1-UBL轴可能在木霉-植物互作诱导系统抗性中起关键作用。
Trichoderma harzianum is a plant-beneficial fungus that secretes small cysteine-rich proteins that induce plant defense responses; however, the molecular mechanism involved in this induction is largely unknown. Here, we report that the class II hydrophobin ThHyd1 acts as an elicitor of induced systemic resistance (ISR) in plants. Immunogold labeling and immunofluorescence revealed ThHyd1 localized on maize (Zea mays) root cell plasma membranes. To identify host plant protein interactors of Hyd1, we screened a maize B73 root cDNA library. ThHyd1 interacted directly with ubiquilin 1-like (UBL). Furthermore, the N-terminal fragment of UBL was primarily responsible for binding with Hyd1 and the eight-cysteine amino acid of Hyd1 participated in the protein-protein interactions. Hyd1 from T. harzianum (Thhyd1) and ubl from maize were co-expressed in Arabidopsis thaliana, they synergistically promoted plant resistance against Botrytis cinerea. RNA-sequencing analysis of global gene expression in maize leaves 24 h after spraying with Curvularia lunata spore suspension showed that Thhyd1-induced systemic resistance was primarily associated with brassinosteroid signaling, likely mediated through BAK1. Jasmonate/ethylene (JA/ET) signaling was also involved to some extent in this response. Our results suggest that the Hyd1-UBL axis might play a key role in inducing systemic resistance as a result of Trichoderma-plant interactions.