Arabidopsis CSLD5 Functions in Cell Plate Formation in a Cell Cycle-Dependent Manner
Arabidopsis CSLD5 Functions in Cell Plate Formation in a Cell Cycle-Dependent Manner
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DOI：
10.1105/tpc.16.00203
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发表时间：
2016-07-01
期刊：
影响因子：
11.6
通讯作者：
Nielsen, Erik
中科院分区：
文献类型：
--
作者：
Gu, Fangwei;Bringmann, Martin;Nielsen, Erik
在植物中，承重细胞壁的存在在细胞分裂过程中提出了独特的挑战。与其他真核生物不同，真核生物在有丝分裂完成后进行收缩胞质分裂，植物相反地合成并组装新的分裂细胞壁，以分离新形成的子细胞。在这里，我们挖掘了拟南芥气孔谱系中单个细胞类型的转录组数据，并确定CSLD5是纤维素合成酶LIKE-D家族的成员，是一种细胞壁生物合成酶，在快速分裂的细胞群中唯一富含。我们进一步证明CSLD5是无言语的直接靶标，它是气孔发育过程中这些分裂的主要转录调节因子。结合遗传分析和荧光标记融合蛋白的体内定位，我们发现CSLD5在植物细胞分裂中优先积累，在那里它参与新形成的细胞板的构建。我们发现CSLD5是一种不稳定的蛋白质，在细胞分裂完成后会迅速降解，并且在ccs52a2突变体中CSLD5的蛋白质周转特性发生了变化，表明CSLD5的周转可能受到细胞周期相关的E3-泛素连接酶-后期促进复合体的调节。
In plants, the presence of a load-bearing cell wall presents unique challenges during cell division. Unlike other eukaryotes, which undergo contractile cytokinesis upon completion of mitosis, plants instead synthesize and assemble a new dividing cell wall to separate newly formed daughter cells. Here, we mine transcriptome data from individual cell types in the Arabidopsis thaliana stomatal lineage and identify CSLD5, a member of the Cellulose Synthase Like-D family, as a cell wall biosynthesis enzyme uniquely enriched in rapidly dividing cell populations. We further show that CSLD5 is a direct target of SPEECHLESS, the master transcriptional regulator of these divisions during stomatal development. Using a combination of genetic analysis and in vivo localization of fluorescently tagged fusion proteins, we show that CSLD5 preferentially accumulates in dividing plant cells where it participates in the construction of newly forming cell plates. We show that CSLD5 is an unstable protein that is rapidly degraded upon completion of cell division and that the protein turnover characteristics of CSLD5 are altered in ccs52a2 mutants, indicating that CSLD5 turnover may be regulated by a cell cycle-associated E3-ubiquitin ligase, the anaphase-promoting complex.