CD68 И СТАБИЛИН-1 ПОЗИТИВНЫЕ МАКРОФАГИ В ПОСТИНФАРКТНОЙ РЕГЕНЕРАЦИИ МИОКАРДА
CD68 И СТАБИЛИН-1 ПОЗИТИВНЫЕ МАКРОФАГИ В ПОСТИНФАРКТНОЙ РЕГЕНЕРАЦИИ МИОКАРДА
复制标题
DOI：
10.15829/1560-4071-2017-11-56-61
复制
发表时间：
2017
影响因子：
--
通讯作者：
А. Э. Гомбожапова;Ю. В. Роговская;М. С. Ребенкова;Ю. Г. Кжышковская;В. В. Рябов-В.
中科院分区：
文献类型：
--
作者：
А. Э. Гомбожапова;Ю. В. Роговская;М. С. Ребенкова;Ю. Г. Кжышковская;В. В. Рябов-В.
瞄准心肌梗死后心脏再生中心脏巨噬细胞群的实验数据转化为临床实践。材料和方法。在这项研究中，包括41例患者，致命的心肌梗死（MI）1型。根据致死性结局时间将所有患者分为4组。结合常规病理组织学检查，对巨噬细胞浸润进行免疫组织化学染色。作为巨噬细胞系的标志物，我们使用CD 68;稳定蛋白-1用于M2巨噬细胞。结果梗死区CD 68+和stabilin-1+巨噬细胞数量增加，在再生期达高峰，后期不再下降。梗死周围区CD 68+巨噬细胞数量在炎症期增加，在修复期达高峰，此后也无明显变化。根据多元回归分析结果，建立了巨噬细胞浸润程度与心肌梗死病程临床指标之间的相关性模型。结论我们的研究将心肌梗死后心脏再生中心脏巨噬细胞亚群的实验结果转化为临床领域。我们观察到心脏巨噬细胞对急性缺血的双相反应，类似于小鼠。再生期stabilin-1+巨噬细胞浸润强度增加。稳定蛋白1+巨噬细胞的数量与MI病程阶段存在显著的强正相关性，这是稳定蛋白-1作为MI患者中M2巨噬细胞的诊断生物标志物的潜在应用的基础。
Aim. Translation of experimental data on cardiac macrophages populations in postinfarction cardiac regeneration, into clinical practice. Material and methods. In the study, 41 patients included, with fatal myocardial infarction (MI) type 1. All patients were selected to 4 groups according to fatal outcome timing. Together with routine pathohistology, immune histochemistry was done, on macrophageal infiltration. As the markers of macrophagal line we used CD68; stabilin-1 was used for M2 macrophages. Results. The amount of CD68+ and stabilin-1+ macrophages in infarction zone increased and reached the peak in regeneratory phase, and did not decline at later stage. In peri-infarction area the amount of CD68+ macrophages increased during inflammatory phase, reached peak at reparation phase and did not change anymore either. By the results of multiple regression, the model was proposed, showing interrelations between the grade of macrophage infiltration and clinical markers of MI course. Conclusion. Our study translates experimental results on cardiac macrophages subpopulations in postinfarction cardiac regeneration into clinical area. We observed a biphasic response of cardiac macrophages on acute ischemia, similar of that in mice. The grade stabilin-1+ macrophagal infiltration intensity increased at regeneration phase. There was significant strong positive correlation of the numbers of stabilin1+ macrophages and MI course phase, that underlies potential application of stabilin-1 as diagnostic biomarker of M2 macrophages in MI patients.