The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses
The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses
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DOI：
10.1083/jcb.200701111
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发表时间：
2007-06-04
影响因子：
7.8
通讯作者：
Ephrussi, Anne
中科院分区：
文献类型：
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作者：
Besse, Florence;Mertel, Sara;Ephrussi, Anne
在可塑性过程和发育过程中，突触的大小及其囊泡释放功能都会经历快速变化。神经元细胞的这一基本特性需要突触膜及其相关细胞骨架的协调重排，但人们对如何实现这种耦合知之甚少。在 GFP 外显子陷阱筛选中，我们鉴定出果蝇 Basigin (Bsg) 是一种含有免疫球蛋白结构域的跨膜蛋白，积聚在神经肌肉接头的周围活动区。突触前和突触后需要 Bsg 来限制突触 bouton 大小，其近膜细胞质残基对该功能很重要。 Bsg 控制突触结构的不同方面，包括突触小泡的分布和突触前皮质肌动蛋白细胞骨架的组织。引人注目的是，突触前末梢还特别需要 bsg 功能来抑制非同步诱发的囊泡释放。因此，我们认为 Bsg 是调节突触区室化和强度以及协调质膜和皮质组织的跨突触复合体的一部分。
Synapses can undergo rapid changes in size as well as in their vesicle release function during both plasticity processes and development. This fundamental property of neuronal cells requires the coordinated rearrangement of synaptic membranes and their associated cytoskeleton, yet remarkably little is known of how this coupling is achieved. In a GFP exon-trap screen, we identified Drosophila melanogaster Basigin (Bsg) as an immunoglobulin domain-containing transmembrane protein accumulating at periactive zones of neuromuscular junctions. Bsg is required pre- and postsynaptically to restrict synaptic bouton size, its juxtamembrane cytoplasmic residues being important for that function. Bsg controls different aspects of synaptic structure, including distribution of synaptic vesicles and organization of the presynaptic cortical actin cytoskeleton. Strikingly, bsg function is also required specifically within the presynaptic terminal to inhibit nonsynchronized evoked vesicle release. We thus propose that Bsg is part of a transsynaptic complex regulating synaptic compartmentalization and strength, and coordinating plasma membrane and cortical organization.