Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling
Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling
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单头凝集素珠阵列有助于全自动糖蛋白分析
DOI：
10.1021/acs.analchem.9b01876
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发表时间：
2019
影响因子：
7.4
通讯作者：
Kuno Atsushi
中科院分区：
文献类型：
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作者：
Shimazaki Hiroko;Saito Kozue;Matsuda Atsushi;Sawakami Kazumi;Kariya Minoru;Segawa Osamu;Miyashita Yukiko;Ueda Tetsuya;Koizuka Michinori;Nakamura Kazuhiro;Kaji Hiroyuki;Tajima Hideji;Kuno Atsushi
疾病中糖改变/差异的定量描述可以导致开发用于体外监测靶糖蛋白变化程度的诊断剂。分析系统沿着组学技术的发展而发展。对于临床实施，它们的完全自动化需要操作简单的设备。在这里，我们报告了一个自动分析系统的糖改变/差异的定量表征，这取决于独特的战略“珠阵列在一个单一的提示。”替代凝集素阵列可以获得纳克量的内源性糖蛋白的聚糖谱的最小表征。一个简单的自动移液机器人可以对聚糖-凝集素相互作用进行精确的化学发光检测，其动态范围较宽，优于基于荧光的凝集素阵列，具有上级。基于尖端的阵列格式能够实现从样品预处理到检测的自动聚糖分析，具有低变化和线性检测，这可以促进这种凝集素阵列在临床实践中的使用。
A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omics-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of “bead arrays in a single tip.” The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan–lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.