Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription-PCR
Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription-PCR
复制标题
DOI：
10.1128/jcm.01704-16
复制
发表时间：
2017-01-01
影响因子：
9.4
通讯作者：
Lim, Chae Seung
中科院分区：
文献类型：
--
作者：
Kim, Young-gon;Yun, Seung Gyu;Lim, Chae Seung
鼻咽拭子(NPSS)被广泛用于呼吸道病毒的多重实时逆转录(RT)-PCR检测。然而，目前尚不清楚NPS样本是否对多重RT-PCR靶标的所有病毒都是最佳的。此外，获取NPS样本的过程会导致大多数患者咳嗽，这可能会增加病毒在医院传播的风险。在这项研究中，收集了236例疑似急性呼吸道疾病的成年男性患者的NPS和唾液样本。用多重实时荧光RT-PCR方法检测16种呼吸道病毒。在236例患者标本中，183例(77.5%)NPS和180例(76.3%)唾液标本至少检出1种呼吸道病毒。鼻咽癌和唾液标本中呼吸道病毒的检出率相当(P=0.766)。共分离到9种病毒349株，其中淋巴组织标本2 5 6株，唾液标本2 73株(P=0.1574)。唾液标本中腺病毒的检出频率较高(P=0.0001)，鼻咽癌标本中甲型流感病毒和人鼻病毒的检出频率较高(P=0.0001和P=0.0289)。通过直接测序排除了从唾液样本中检测到假阳性腺病毒的可能性。总而言之，这两种采样方法都不总是比另一种更敏感。我们认为，这些检测NPS-唾液混合样本中呼吸道病毒的经济有效的方法可能对未来的研究有价值。
Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes coughing in most patients, which possibly increases the risk of nosocomial spread of viruses. In this study, paired NPS and saliva specimens were collected from 236 adult male patients with suspected acute respiratory illnesses. Specimens were tested for 16 respiratory viruses by multiplex real-time RT-PCR. Among the specimens collected from the 236 patients, at least 1 respiratory virus was detected in 183 NPS specimens (77.5%) and 180 saliva specimens (76.3%). The rates of detection of respiratory viruses were comparable for NPS and saliva specimens (P = 0.766). Nine virus species and 349 viruses were isolated, 256 from NPS specimens and 273 from saliva specimens (P = 0.1574). Adenovirus was detected more frequently in saliva samples (P = 0.0001), whereas influenza virus type A and human rhinovirus were detected more frequently in NPS specimens (P = 0.0001 and P = 0.0289, respectively). The possibility of false-positive adenovirus detection from saliva samples was excluded by direct sequencing. In conclusion, neither of the sampling methods was consistently more sensitive than the other. We suggest that these cost-effective methods for detecting respiratory viruses in mixed NPS-saliva specimens might be valuable for future studies.