Nitrate removal and biofilm characteristics in methanotrophic membrane biofilm reactors with various gas supply regimes.
Nitrate removal and biofilm characteristics in methanotrophic membrane biofilm reactors with various gas supply regimes.
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DOI：
10.1016/j.watres.2009.09.009
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发表时间：
2010
期刊：
影响因子：
12.8
通讯作者：
O. Modin;K. Fukushi;F. Nakajima;Kazuo Yamamoto
中科院分区：
文献类型：
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作者：
O. Modin;K. Fukushi;F. Nakajima;Kazuo Yamamoto
好氧甲烷氧化菌可以通过同化还原和产生可供共存反硝化菌利用的可溶性有机物，有助于从污染水、废水或垃圾渗滤液中去除硝酸盐。本研究的目的是研究具有不同氧气和甲烷气体供应方式的膜生物膜反应器 (MBfR) 中的硝酸盐去除和生物膜特性。开发了三种 MBfR 配置，与之前在悬浮培养物中观察到的结果相比，它们在甲烷利用率（值范围从 0.25 至 0.36molNmol−1CH4）方面实现了显着更高的硝酸盐去除效率。研究了两种具有不同供氧模式的 MBfR 的生物膜特性。生物膜的结构不同，但两者都以靠近膜表面生长的 I 型甲烷氧化菌为主。亚硝酸还原酶基因 nirS 和 nirK 的检测表明，混合培养生物膜中存在反硝化的遗传潜力。
Aerobic methanotrophs can contribute to nitrate removal from contaminated waters, wastewaters, or landfill leachate by assimilatory reduction and by producing soluble organics that can be utilized by coexisting denitrifiers. The goal of this study was to investigate nitrate removal and biofilm characteristics in membrane biofilm reactors (MBfR) with various supply regimes of oxygen and methane gas. Three MBfR configurations were developed and they achieved significantly higher nitrate removal efficiencies in terms of methane utilization (values ranging from 0.25 to 0.36molNmol−1CH4) than have previously been observed with suspended cultures. The biofilm characteristics were investigated in two MBfRs with varying modes of oxygen supply. The biofilms differed in structure, but both were dominated by Type I methanotrophs growing close to the membrane surface. Detection of the nitrite reductase genes, nirS and nirK, suggested genetic potential for denitrification was present in the mixed culture biofilms.