Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei
Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei
复制标题
DOI：
10.1016/j.jsb.2012.01.009
复制
发表时间：
2012-05-01
影响因子：
3
通讯作者：
Gull, Keith
中科院分区：
文献类型：
--
作者：
Hoeoeg, Johanna L.;Bouchet-Marquis, Cedric;Gull, Keith
布氏锥虫是一种单细胞原生生物，可引起非洲昏睡病。这些寄生虫有一个鞭毛，附着在细胞体上，是其运动不可或缺的。鞭毛由一个典型的9 + 2轴丝和一个旁鞭毛杆（PFR），一个复杂的三部分，纤维结构，连接到轴丝。在本文中，我们描述了冷冻电子断层扫描的结果未受干扰的鞭毛。这种方法揭示了可能参与将鞭毛附着到细胞的新结构。我们还展示了第一个低温电子断层扫描图像的基体原位，揭示电子致密结构内的三重微管。PFR的子断层图像平均显示其远端区域被组织为斜方晶体。(C)2012 Elsevier Inc. All rights reserved.
Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9 + 2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal. (C) 2012 Elsevier Inc. All rights reserved.