Immunochemical study on basement membrane (type IV) collagens.
Immunochemical study on basement membrane (type IV) collagens.
复制标题
基底膜（IV 型）胶原蛋白的免疫化学研究。
作者：
R. Timpl;R. Glanville;G. Wick;G. Martin
基底膜（IV型）胶原蛋白提取小鼠肿瘤与乙酸和人胎盘后，有限的酶消化。在兔和豚鼠中产生抗两种胶原的抗血清，并通过各种测定进行检查。发现这些抗血清对基底膜胶原蛋白具有特异性，并且与I、II和III型间质胶原蛋白或与由α A和α B链组成的人胎盘胶原蛋白几乎没有交叉反应。不同程度的交叉反应之间观察到抗血清的人和小鼠IV型胶原蛋白。免疫化学分析表明，存在三个不同的决定因素在肿瘤IV型胶原蛋白。兔抗此抗原的抗血清与抗胶原酶片段或与胶原二硫化物结合片段（P3）反应。豚鼠抗血清主要识别P3片段中的抗原决定簇。抗胎盘IV型胶原蛋白的血清与另一种胶原蛋白，缺乏二硫键的胃蛋白酶片段（P1）反应。这些抗血清与从人胎盘胃蛋白酶消化物和牛透镜囊制备的胶原α 1（IV）链显示完全交叉反应。
Basement membrane (type IV) collagens were extracted from a mouse tumour with acetic acid and from human placenta after limited enzymatic digestion. Antisera were produced against both collagens in rabbits and guinea-pigs and examined by various assays. These antisera were found to be specific for basement membrane collagen and showed little or no cross-reactions with the interstitial collagens, types I, II and III or with human placenta collagen consisting of alpha A and alpha B chains. Varying degrees of cross-reaction were observed between antisera to human and mouse type IV collagen. Immunochemical analyses demonstrated the presence of three distinct determinants in the tumour type IV collagen. Rabbit antisera against this antigen reacted with either collagenase-resistant segments or with a collagenous, disulphide-bonded segment (P3). Guinea-pig antisera recognized primarily antigenic determinants in the P3 segment. Antisera to placenta type IV collagen reacted with another collagenous, pepsin fragment (P1) which lacks disulphide bonds. These antisera showed complete cross-reaction with collagenous alpha 1 (IV) chains prepared from pepsin-digests of human placenta and bovine lens capsule.