Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass
Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass
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DOI：
10.3390/ijms21051628
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Tsuchida, Kunihiro
中科院分区：
文献类型：
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作者：
Hitachi, Keisuke;Nakatani, Masashi;Tsuchida, Kunihiro
骨骼肌是一种高度可塑性的器官，对人体的动态平衡和健康是必不可少的。骨骼肌的大小随着内在和外在刺激的变化而变化。尽管包括肌肉抑制素、核因子-kappaβ和胰岛素样生长因子-1在内的蛋白质编码RNA在决定骨骼肌质量方面起着关键作用，但长非编码RNA(LncRNAs)在骨骼肌质量调节中的作用仍有待阐明。在这里，我们对9个与骨骼肌分化相关的lncRNAs(DRR、DUM1、Linc-MD1、Linc-YY1、LncMyod、Neat1、Myoparr、MALAT1和SRA)和3个基因组印记相关的lncRNAs(Gtl2、H19和IG-DMR)进行了表达谱分析。用定量RT-PCR方法检测了6种骨骼肌萎缩模型(失神经支配、铸型、尾部悬吊、地塞米松应用、癌症恶病质和禁食)和2种骨骼肌肥大模型(机械负荷过重和肌肉生长抑制素基因缺陷)中这些lncRNAs的表达水平。对这些lncRNA表达水平的聚类分析被成功地用于将肌肉萎缩模型分为两个亚组。此外，Gtl2、IG-DMR和DUM1的表达随着骨骼肌大小的变化而改变。对LncRNAs在多种肌肉萎缩和肥大模型中的表达水平的综述为了解LncRNAs在决定骨骼肌质量中的作用提供了新的视角。
Skeletal muscle is a highly plastic organ that is necessary for homeostasis and health of the human body. The size of skeletal muscle changes in response to intrinsic and extrinsic stimuli. Although protein-coding RNAs including myostatin, NF-kappa beta, and insulin-like growth factor-1 (IGF-1), have pivotal roles in determining the skeletal muscle mass, the role of long non-coding RNAs (lncRNAs) in the regulation of skeletal muscle mass remains to be elucidated. Here, we performed expression profiling of nine skeletal muscle differentiation-related lncRNAs (DRR, DUM1, linc-MD1, linc-YY1, LncMyod, Neat1, Myoparr, Malat1, and SRA) and three genomic imprinting-related lncRNAs (Gtl2, H19, and IG-DMR) in mouse skeletal muscle. The expression levels of these lncRNAs were examined by quantitative RT-PCR in six skeletal muscle atrophy models (denervation, casting, tail suspension, dexamethasone-administration, cancer cachexia, and fasting) and two skeletal muscle hypertrophy models (mechanical overload and deficiency of the myostatin gene). Cluster analyses of these lncRNA expression levels were successfully used to categorize the muscle atrophy models into two sub-groups. In addition, the expression of Gtl2, IG-DMR, and DUM1 was altered along with changes in the skeletal muscle size. The overview of the expression levels of lncRNAs in multiple muscle atrophy and hypertrophy models provides a novel insight into the role of lncRNAs in determining the skeletal muscle mass.