Novel strategy for treatment of inner ears using a biodegradable gel
Novel strategy for treatment of inner ears using a biodegradable gel
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DOI：
10.1097/01.mlg.0000183020.32435.59
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发表时间：
2005-11-01
期刊：
影响因子：
2.6
通讯作者：
Ito, J
中科院分区：
文献类型：
--
作者：
Endo, T;Nakagawa, T;Ito, J
目标。本研究旨在评估可生物降解水凝胶作为内耳药物传递介质的功效。选择脑源性神经营养因子（BDNF）作为给药剂。方法：首先，我们将含有BDNF的水凝胶放置在耳圆窗膜上，使用酶联免疫吸附法测量耳蜗液中BDNF的浓度。其次，采用SGN变性动物模型，研究水凝胶应用BDNF对听觉初级神经元（螺旋神经节神经元[SGN]）的功能和组织学保护作用。结果：BDNF通过水凝胶的方式持续进入耳蜗液。其次，采用听觉原代神经元（SGN）变性动物模型，研究水凝胶注入BDNF对其功能和组织学的保护作用。电诱发的听觉-脑干反应的测量表明，通过水凝胶递送的BDNF显著降低了阈值升高。神经丝200 kD的免疫组化显示，通过水凝胶应用BDNF增加了sgn的存活。结论：生物可降解水凝胶可用于内耳给药。
Objective. The present study aimed to evaluate the efficacy of a biodegradable hydrogel as a drug-delivery medium for the inner ear. Brain-derived neurotrophic factor (BDNF) was chosen as the agent to be administered. Method: First, we used an enzyme-linked immunosorbent assay to measure BDNF concentrations in the cochlear fluid after placing a hydrogel containing this agent onto the round-window membrane of the ear. Second, the functional and histologic protection of the auditory primary neurons (spiral ganglion neurons [SGNs]) by BDNF applied through the hydrogel was examined using an animal model of SGN degeneration. Results: The results revealed sustained delivery of BDNF into the cochlear fluid by way of the hydrogel. Second, the functional and histologic protection of the auditory primary neurons (SGNs) by BDNF applied through the hydrogel was examined using an animal model of SGN degeneration. The measurement of electrically evoked auditory-brainstem responses demonstrated that BDNF delivered by way of the hydrogel significantly reduced the threshold elevation. Immunohistochemistry for neurofilament 200 kD demonstrated increased survival of SGNs because of BDNF application through the hydrogel. Conclusion: These findings indicate that biodegradable hydrogels can be used for drug delivery to the inner ear.