Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
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小鼠和中国仓鼠的次黄嘌呤鸟嘌呤磷酸核糖基转移酶基因：cDNA重组体的构建和序列分析。
DOI：
10.1093/nar/10.21.6763
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发表时间：
1982
影响因子：
14.9
通讯作者：
Chinault,AC
中科院分区：
文献类型：
--
作者：
Konecki,DS;Brennand,J;Fuscoe,JC;Caskey,CT;Chinault,AC
已从cDNA文库中分离出含有与编码小鼠和中国仓鼠细胞系的次黄嘌呤-鸟嘌呤磷酸核糖基转移酶（HPRT）的mRNA互补的DNA插入片段的重组质粒，并通过DNA序列分析进行了表征。小鼠cDNA序列全长1292个核苷酸，中国仓鼠cDNA序列全长1301个核苷酸。这些序列中的每一个包括对应于HPRT蛋白编码区的654个核苷酸（218个氨基酸）的开放阅读框。推导的氨基酸序列的小鼠和中国仓鼠酶，并与人HPRT。至少95%的氨基酸在三个物种中是保守的。此外，我们提出的证据表明，两个物种的HPRT mRNA，这是利用在RNA转录的加工过程中的多聚腺苷酸化的网站不同，存在于中国仓鼠细胞。
Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine–guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDNA sequence and 1301 nucleotides of the Chinese hamster cDNA sequence has been determined. Each of these sequences includes an open reading frame of 654 nucleotides (218 amino acids) corresponding to the HPRT protein coding region. The deduced amino acid sequences for the mouse and Chinese hamster enzymes are presented and compared to that of human HPRT. At least 95% of the amino acids are conserved in the three species. In addition, we present evidence that two species of HPRT mRNA, which differ in the site of polyadenylation that is utilized during processing of the RNA transcripts, exist in Chinese hamster cells.
体细胞遗传学
影响因子：
64.8
作者：
H. Harris
通讯作者：
H. Harris