Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco
Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco
复制标题
花生 CLAVATA1 样富含亮氨酸的重复受体样激酶 AhRLK1 的过度表达可增强烟草对青枯病的抗性
DOI：
10.1093/jxb/erz274
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发表时间：
2019
影响因子：
6.9
通讯作者：
Zhuang Wei Jian
中科院分区：
文献类型：
--
作者：
Zhang Chong;Chen Hua;Zhuang Rui Rong;Chen Yu Ting;Deng Ye;Cai Tie Cheng;Wang Shuai Yin;Liu Qin Zheng;Tang Rong Hua;Shan Shi Hua;Pan Rong Long;Chen Li Song;Zhuang Wei Jian
青枯病是一种危害数百种植物的毁灭性病害，但其寄主因子的研究却很少。富含亮氨酸重复序列的受体样激酶基因AhRLK1（CLAVATA 1）在花生中被发现在接种R.青枯菌属AhRLK1蛋白定位于细胞膜和细胞壁。qPCR结果表明，在感病品种中，AhRLK1被诱导表达，而在抗病品种中，AhRLK1的表达变化不大。青枯菌属激素如水杨酸、脱落酸、茉莉酸甲酯和乙烯利诱导AhRLK1表达。而在低温和干旱处理下，AhRLK1的表达下调。AhRLK1的瞬时过表达导致本氏烟草的过敏反应（HR）。此外，AhRLK1在烟草中的过表达显著增加了对R的抗性。青枯菌属此外，在转基因株系中，HR和激素信号通路中的多数代表性防御反应基因的转录量均显著增加，其中R基因信号通路中的EDS1和PAD 4表达上调，NDR1表达下调。因此，AhRLK1可能会增加对R的防御反应。青枯菌通过HR和激素防御信号，特别是通过EDS1途径的R基因信号传导。这些结果为CLAVATA1的功能提供了新的认识，为花生的遗传改良提供了理论依据。
Bacterial wilt caused byRalstonia solanacearumis a devastating disease affecting hundreds of plant species, yet the host factors remain poorly characterized. The leucine-rich repeat receptor-like kinase geneAhRLK1, characterized asCLAVATA1, was found to be up-regulated in peanut upon inoculation withR. solanacearum. The AhRLK1 protein was localized in the plasma membrane and cell wall. qPCR results showedAhRLK1was induced in a susceptible variety but little changed in a resistant cultivar after inoculated withR. solanacearum. Hormones such as salicylic acid, abscisic acid, methyl jasmonate, and ethephon inducedAhRLK1expression. In contrast,AhRLK1expression was down-regulated under cold and drought treatments. Transient overexpression ofAhRLK1led to a hypersensitive response (HR) inNicotiana benthamiana. Furthermore,AhRLK1overexpression in tobacco significantly increased the resistance toR. solanacearum. Besides, the transcripts of most representative defense responsive genes in HR and hormone signal pathways were significantly increased in the transgenic lines.EDS1andPAD4in the R gene signaling pathway were also up-regulated, butNDR1was down-regulated. Accordingly,AhRLK1may increase the defense response toR. solanacearumvia HR and hormone defense signaling, in particular through theEDS1pathway of R gene signaling. These results provide a new understanding of theCLAVATA1function and will contribute to genetic enhancement of peanut.