Reversible photopadlocking on double-stranded DNA

Reversible photopadlocking on double-stranded DNA
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DOI:
10.1039/b707524c
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发表时间:
2007-01-01
影响因子:
4.9
通讯作者:
Saito, Isao
Saito, Isao
中科院分区:
化学2区
文献类型:
--
作者:
Fujimoto, Kenzo;Matsuda, Shigeo;Saito, Isao

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我们描述了一种在双链 DNA 模板上进行寡核苷酸 (ODN) 可逆光环化的高效方法:含有 5-羧基乙烯基-29-脱氧尿苷的 ODN 在双链质粒 DNA 的靶序列周围可逆环化,从而形成连接的质粒。
We describe a highly efficient method for reversible photo-circularization of oligonucleotide (ODN) on a double-stranded DNA template: 5-carboxyvinyl-29-deoxyuridine-containing ODN was reversibly circularized around the target sequence of the double-stranded plasmid DNA resulting in formation of a catenated plasmid.