Reversible photopadlocking on double-stranded DNA
Reversible photopadlocking on double-stranded DNA
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DOI:
10.1039/b707524c
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发表时间:
2007-01-01
影响因子:
4.9
通讯作者:
Saito, Isao
中科院分区:
文献类型:
--
作者:
Fujimoto, Kenzo;Matsuda, Shigeo;Saito, Isao
We describe a highly efficient method for reversible photo-circularization of oligonucleotide (ODN) on a double-stranded DNA template: 5-carboxyvinyl-29-deoxyuridine-containing ODN was reversibly circularized around the target sequence of the double-stranded plasmid DNA resulting in formation of a catenated plasmid.