RNA-Seq transcriptomic analysis of the Morus alba L. leaves exposed to high-level UVB with or without dark treatment
RNA-Seq transcriptomic analysis of the Morus alba L. leaves exposed to high-level UVB with or without dark treatment
复制标题
对暴露于高水平 UVB（有或没有暗处理）的桑树叶进行 RNA-Seq 转录组分析
DOI：
10.1016/j.gene.2017.12.045
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发表时间：
2018-03-01
期刊：
影响因子：
3.5
通讯作者：
Tian, Jingkui
中科院分区：
文献类型：
--
作者：
Guan, Qijie;Yu, Jiaojiao;Tian, Jingkui
紫外线 B (UVB) 照射会产生过多的活性氧，从而诱发植物细胞氧化应激。 Mores alba L. (M. abla) 是一种用于治疗人类疾病的重要药用植物。此外，它的叶子也被广泛用作蚕的食物。在我们之前的研究中，我们发现高水平的 UVB 照射和暗培养会导致 M.able 叶子中次生代谢产物的积累。本研究的目的是描述和比较不同处理（对照、UVB、UVB + 暗）的白杨叶转录组学。使用 Illumina Hiseq 2000 系统对 M. alba 的叶转录本进行测序，该系统产生了 14.27 Gb 的数据，包括三个文库中的 153,204,462 个双端读数。我们从头组装了 133,002 个转录本，平均长度为 1270 bp，并过滤了 69,728 个非冗余 unigene。对非冗余国家生物技术信息中心 (NCBI) 蛋白质数据库进行相似性搜索，结果返回 41.08% 的命中率。在UniProtKB/SwissProt数据库注释的20,040个unigenes中，16,683个unigenes被分配了102,232个基因本体术语，并且在287个已知代谢途径中鉴定了6667个unigenes。差异基因表达分析和实时定量PCR测试的结果表明，暗孵育下的UVB照射增强了白花叶中黄酮类化合物的生物合成。我们的研究结果为更好地了解银花叶在非生物胁迫下的代谢机制提供了有价值的证据。
Ultraviolet-B (UVB) irradiation induces oxidative stress in plant cells due to the generation of excessive reactive oxygen species. Mores alba L. (M. abla) is an important medicinal plant used for the treatment of human diseases. Also, its leaves are widely used as food for silkworms. In our previous research, we found that a high level of UVB irradiation with dark incubation led to the accumulation of secondary metabolites in M. able leaf. The aim of the present study was to describe and compare M. alba leaf transcriptomics with different treatments (control, UVB, UVB + dark). Leaf transcripts from M. alba were sequenced using an Illumina Hiseq 2000 system, which produced 14.27 Gb of data including 153,204,462 paired-end reads among the three libraries. We de novo assembled 133,002 transcripts with an average length of 1270 bp and filtered 69,728 non-redundant unigenes. A similarity search was performed against the non-redundant National Center of Biotechnology Information (NCBI) protein database, which returned 41.08% hits. Among the 20,040 unigenes annotated in UniProtKB/SwissProt database, 16,683 unigenes were assigned 102,232 gene ontology terms and 6667 unigenes were identified in 287 known metabolic pathways. Results of differential gene expression analysis together with real-time quantitative PCR tests indicated that UVB irradiation with dark incubation enhanced the fiavonoid biosynthesis in M. alba leaf. Our findings provided a valuable proof for a better understanding of the metabolic mechanism under abiotic stresses in M. alba leaf.