The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum.
The Histone Chaperone Network Is Highly Conserved in Physarum polycephalum.
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DOI：
10.3390/ijms24021051
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发表时间：
2023-01-05
影响因子：
5.6
通讯作者：
Duc, Celine
中科院分区：
文献类型：
--
作者：
Poulet, Axel;Rousselot, Ellyn;Teletchea, Stephane;Noirot, Celine;Jacob, Yannick;van Wolfswinkel, Josien;Thiriet, Christophe;Duc, Celine
核小体由组蛋白和DNA组成。在它们沉积在染色质上之前，组蛋白被称为组蛋白分子伴侣的专门和多样的蛋白质屏蔽。它们在组蛋白的整个细胞生命过程中护送组蛋白，并确保其正确掺入染色质中。多头绒泡菌（Physarum polycephalum）是一种菌体动物，位于真核生物树的冠部。我们以前发现，组蛋白，这是高度保守的植物和动物之间，也是高度保守的绒泡菌。然而，组蛋白伴侣在动物界和植物界之间存在显著差异，这为我们进一步研究绒泡菌属中组蛋白伴侣的保守性及其相对于动物和植物的进化提供了线索。大多数已知的组蛋白伴侣及其功能结构域是保守的，也是组蛋白和伴侣相互作用所需的关键残基。绒泡菌属与酵母、植物和动物不同，但PpHIRA、PpCABIN1和PpSPT6在结构上与植物直向同源物相似。PpFACT与酵母复合体密切相关，并且绒泡菌基因组编码动物特异性APFL分子伴侣。此外，我们进行了RNA测序，以监测分子伴侣在细胞周期中的表达，并在S期发现了两种不同的模式。总之，我们的研究证明了组蛋白伴侣在处理早期分支真核生物中的组蛋白中的保守作用。
The nucleosome is composed of histones and DNA. Prior to their deposition on chromatin, histones are shielded by specialized and diverse proteins known as histone chaperones. They escort histones during their entire cellular life and ensure their proper incorporation in chromatin. Physarum polycephalum is a Mycetozoan, a clade located at the crown of the eukaryotic tree. We previously found that histones, which are highly conserved between plants and animals, are also highly conserved in Physarum. However, histone chaperones differ significantly between animal and plant kingdoms, and this thus probed us to further study the conservation of histone chaperones in Physarum and their evolution relative to animal and plants. Most of the known histone chaperones and their functional domains are conserved as well as key residues required for histone and chaperone interactions. Physarum is divergent from yeast, plants and animals, but PpHIRA, PpCABIN1 and PpSPT6 are similar in structure to plant orthologues. PpFACT is closely related to the yeast complex, and the Physarum genome encodes the animal-specific APFL chaperone. Furthermore, we performed RNA sequencing to monitor chaperone expression during the cell cycle and uncovered two distinct patterns during S-phase. In summary, our study demonstrates the conserved role of histone chaperones in handling histones in an early-branching eukaryote.
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影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
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影响因子：
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DOI：
10.1074/jbc.m111.223453
发表时间：
2011-05-20
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Alvarez F;Muñoz F;Schilcher P;Imhof A;Almouzni G;Loyola A
通讯作者：
Loyola A
DOI：
10.1073/pnas.2100178118
发表时间：
2021-08-03
影响因子：
11.1
作者：
Armstrong C;Spencer SL
通讯作者：
Spencer SL