Epithelial Functions of the Residual Bowel After Surgery for Necrotising Enterocolitis in Human Infants
Epithelial Functions of the Residual Bowel After Surgery for Necrotising Enterocolitis in Human Infants
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DOI：
10.1097/mpg.0b013e318186d341
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Renes, Ingrid B.
中科院分区：
文献类型：
--
作者：
Schaart, Maaike W.;de Bruijn, Adrianus C. J. M.;Renes, Ingrid B.
目的：关于人类婴儿坏死性小肠结肠炎（NEC）切除术后残留小肠或结肠上皮功能的信息很少。我们的目的是评估在肠切除术急性NEC和连续吻合口closure.Materials and Methods：上皮细胞形态，增殖和蛋白质表达的肠切除边缘的组织上皮功能进行了（免疫）histochemics.Results：急性NEC与严重和轻度上皮细胞损伤不同上皮损失相当不受影响的上皮。在急性NEC和造口关闭时，上皮细胞增殖均增加。在急性NEC中，乳糖酶。在严重受累的上皮中，葡萄糖转运蛋白-2和-5的表达下调，而蔗糖酶-异麦芽糖酶和肠脂肪酸结合蛋白的表达保持不变。杯状细胞继续表达粘蛋白2和三叶因子3，但数量减少。此外，在急性NEC，潘氏细胞弱溶菌酶阳性，数量减少。在气孔关闭，上述细胞类型的特异性标志物的表达已完全reestablished.Conclusions：切除急性NEC后的残留肠上皮细胞增殖/分化平衡紊乱。急性NEC与不同肠细胞特异性蛋白的下调有关。由于杯状细胞和潘氏细胞在急性NEC中的损失，粘膜屏障和防御功能可能受损。JPGN 49：31-41，2009.
Objectives: Information on epithelial functions of the residual small or colonic bowel after resection for necrotising enterocolitis (NEC) in human infants is scarce. Our aim is to evaluate epithelial functions in the intestinal resection margins of tissue obtained at bowel resection for acute NEC and consecutive stoma closure.Materials and Methods: Epithelial morphology, proliferation, and protein expression were (immuno)histochemically studied.Results: Acute NEC was associated with severe and mild epithelial damage varying from epithelial loss to fairly unaffected epithelium. Epithelial proliferation was increased both at acute NEC and at stoma closure. In acute NEC, lactase. glucose transporter-2 and -5 expression was down-regulated in severely affected epithelium, whereas sucrase-isomaltase and intestinal fatty acid binding protein expression was maintained. Goblet cells continued to express mucin 2 and trefoil factor 3, however, their numbers were decreased. Moreover, in acute NEC, Paneth cells were weakly lysozyme positive and were reduced in number. At stoma closure, expression of the above cell type-specific markers had completely been re-established.Conclusions: Residual bowel after resection for acute NEC shows a disturbed epithelial proliferation/differentiation balance. Acute NEC was associated with downregulation of distinct enterocyte-specific proteins. Because of goblet cell and Paneth cell loss in acute NEC, mucosal barrier, and defense functions may be impaired. JPGN 49:31-41, 2009.