Comparative mitochondrial genome analysis reveals the evolutionary rearrangement mechanism in Brassica
Comparative mitochondrial genome analysis reveals the evolutionary rearrangement mechanism in Brassica
复制标题
比较线粒体基因组分析揭示了芸苔属的进化重排机制
DOI：
10.1111/plb.12414
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发表时间：
2016-05-01
期刊：
影响因子：
3.9
通讯作者：
Zhang, M.
中科院分区：
文献类型：
--
作者：
Yang, J.;Liu, G.;Zhang, M.
芸苔属有许多种类，是重要的油，蔬菜和其他食品。三个线粒体基因组类型（mitotype）起源于其共同的祖先。本论文通过从头组装的方法获得了一个长为232，407 bp的黑B.nigra线粒体主环基因组。同线分析表明，白菜和甘蓝的线粒体基因组具有比黑芥更好的同线关系。通过主成分分析和系统发育树的构建，确定了芸苔属U三角区3个异源四倍体物种的母系祖先。在异源四倍体B.napus中发现了多样的有丝分裂型，其中napus型B.napus来源于B.oleracea，而polima型B.napus则来源于B. rapa。此外，油菜型B.napus的线粒体基因组更接近于葡萄孢型而不是甘蓝型。几个线粒体基因的亚化学计量移位表明，在驯化和/或植物育种过程中，线粒体基因组重排经历了进化选择。我们的研究结果阐明了二倍体物种在芸苔属异源四倍体物种的母系起源中的作用，并提出了线粒体基因组育种选择的可能性。
The genus Brassica has many species that are important for oil, vegetable and other food products. Three mitochondrial genome types (mitotype) originated from its common ancestor. In this paper, a B.nigra mitochondrial main circle genome with 232,407bp was generated through de novo assembly. Synteny analysis showed that the mitochondrial genomes of B.rapa and B.oleracea had a better syntenic relationship than B.nigra. Principal components analysis and development of a phylogenetic tree indicated maternal ancestors of three allotetraploid species in Us triangle of Brassica. Diversified mitotypes were found in allotetraploid B.napus, in which napus-type B.napus was derived from B.oleracea, while polima-type B.napus was inherited from B.rapa. In addition, the mitochondrial genome of napus-type B.napus was closer to botrytis-type than capitata-type B.oleracea. The sub-stoichiometric shifting of several mitochondrial genes suggested that mitochondrial genome rearrangement underwent evolutionary selection during domestication and/or plant breeding. Our findings clarify the role of diploid species in the maternal origin of allotetraploid species in Brassica and suggest the possibility of breeding selection of the mitochondrial genome.