IDENTIFICATION OF 3,4-DIHYDROXYPHENYLALANINE, 5,6-DIHYDROXYINDOLE, AND N-ACETYLARTERENONE DURING EUMELANIN FORMATION IN IMMUNE REACTIVE LARVAE OF DROSOPHILA-MELANOGASTER
IDENTIFICATION OF 3,4-DIHYDROXYPHENYLALANINE, 5,6-DIHYDROXYINDOLE, AND N-ACETYLARTERENONE DURING EUMELANIN FORMATION IN IMMUNE REACTIVE LARVAE OF DROSOPHILA-MELANOGASTER
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DOI：
10.1002/arch.940200303
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发表时间：
1992-01-01
影响因子：
2.2
通讯作者：
FREY, F
中科院分区：
文献类型：
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作者：
NAPPI, AJ;VASS, E;FREY, F
通过电化学方法在被黄蜂 Leptopilina boulardi 寄生后的黑腹果蝇免疫反应幼虫的血淋巴中检测到 3,4-二羟基苯丙氨酸、5-6-二羟基吲哚和 N-乙酰黄芪酮。通过反相离子对高压液相色谱和电化学检测分离后，对儿茶酚进行测定。 5,6-二羟基吲哚的存在明确地建立了果蝇防御反应中的真黑素途径，并证实了先前的研究表明某些儿茶酚胺代谢酶与昆虫免疫有关。 N-乙酰蒿酮（主要硬化剂 N-乙酰多巴胺的衍生物）的出现，证实了醌甲基化物异构酶在儿茶酚胺代谢调节中的存在和拟议的参与，并表明果蝇在针对寄生虫的免疫反应中形成的细胞囊很可能是真黑素和硬化素的复合物。免疫反应宿主的血淋巴样本中不存在 3,4-二羟基苯乙酸，这表明在寄生过程中，某些儿茶酚胺和代谢前体可能会在替代途径中重新利用，其中一些可能用于防御反应。
3,4-Dihydroxyphenylalanine,5-6-dihydroxyindole, and N-acetylarterenone were detected by electrochemical methods in the hemolymph of immune reactive larvae of Drosophila melanogaster following parasitization by the wasp Leptopilina boulardi. Determinations of the catechols were made after separation by reverse phase, ion-pairing high pressure liquid chromatography with electrochemical detection. The presence of 5,6-dihydroxyindole unequivocally establishes the eumelanin pathway in the defense reponse of Drosophila, and confirms previous investigations which have implicated certain catecholamine metabolizing enzymes in insect immunity. The occurrence of N-acetylarterenone, a derivative of the principal sclerotizing agent N-acetyldopamine, verifies the existence and proposed involvement of quinone methide isomerase in the regulation of catecholamine metabolism, and suggests that the cellular capsule formed by Drosophila in immune reactions against parasites is most likely a composite of both eumelanin and sclerotin. The absence of 3,4-dihydroxyphenylacetic acid in hemolymph samples from immune reactive hosts suggests that during parasitization certain catecholamines and metabolic precursors may be re-employed in alternate pathways, some of which may be used in defense reactions.