The Linker Histone Plays a Dual Role during Gametogenesis in Saccharomyces cerevisiae
The Linker Histone Plays a Dual Role during Gametogenesis in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.00282-12
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发表时间：
2012-07-01
影响因子：
5.3
通讯作者：
Berger, Shelley L.
中科院分区：
文献类型：
--
作者：
Bryant, Jessica M.;Govin, Jerome;Berger, Shelley L.
配子的分化涉及染色质的显著变化，影响转录、减数分裂和细胞形态。酿酒酵母的孢子形成与精子发生有许多共同的染色质特征，包括细胞核的10倍致密化。为了确定新的蛋白质参与孢子核组织，我们纯化染色质成熟孢子，发现一个显着丰富的连接组蛋白（HHO 1）。Hho 1的功能已被证明是难以捉摸的营养生长过程中，但在这里，我们证明了它的要求，有效的孢子形成和孢子基因组的完全压实。Hho 1染色质免疫沉淀，然后测序（ChIP-seq）显示，在成熟孢子中增加的全基因组结合，并提供了新的体内证据的接头组蛋白结合到核小体接头DNA。我们还将Hho 1的功能与转录因子Ume 6（早期减数分裂基因的主阻遏物）联系起来。Hho 1和Ume 6在减数分裂过程中耗尽，并在营养生长过程中获得的已发表的ChIP芯片数据的分析揭示了这两种蛋白质在早期减数分裂基因的启动子处的高结合相关性。此外，Ume 6促进Hho 1与减数分裂基因启动子的结合。因此，Hho 1可能在孢子形成过程中发挥双重作用：Hho 1和Ume 6消耗促进减数分裂的开始，通过激活Ume 6抑制的早期减数分裂基因，而Hho 1丰富的成熟孢子有助于孢子基因组压实。
The differentiation of gametes involves dramatic changes to chromatin, affecting transcription, meiosis, and cell morphology. Sporulation in Saccharomyces cerevisiae shares many chromatin features with spermatogenesis, including a 10-fold compaction of the nucleus. To identify new proteins involved in spore nuclear organization, we purified chromatin from mature spores and discovered a significant enrichment of the linker histone (Hho1). The function of Hho1 has proven to be elusive during vegetative growth, but here we demonstrate its requirement for efficient sporulation and full compaction of the spore genome. Hho1 chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed increased genome-wide binding in mature spores and provides novel in vivo evidence of the linker histone binding to nucleosomal linker DNA. We also link Hho1 function to the transcription factor Ume6, the master repressor of early meiotic genes. Hho1 and Ume6 are depleted during meiosis, and analysis of published ChIP-chip data obtained during vegetative growth reveals a high binding correlation of both proteins at promoters of early meiotic genes. Moreover, Ume6 promotes binding of Hho1 to meiotic gene promoters. Thus, Hho1 may play a dual role during sporulation: Hho1 and Ume6 depletion facilitates the onset of meiosis via activation of Ume6-repressed early meiotic genes, whereas Hho1 enrichment in mature spores contributes to spore genome compaction.