miR-29a-3p promotes migration and invasion in ameloblastoma via Wnt/β-catenin signaling by targeting catenin beta interacting protein 1
miR-29a-3p promotes migration and invasion in ameloblastoma via Wnt/β-catenin signaling by targeting catenin beta interacting protein 1
复制标题
miR-29a-3p通过靶向连环蛋白β相互作用蛋白1通过Wnt/β-连环蛋白信号促进成釉细胞瘤的迁移和侵袭
DOI：
10.1002/hed.26888
复制
发表时间：
2021-10-12
影响因子：
2.9
通讯作者：
Zhong，Ming
中科院分区：
文献类型：
--
作者：
Liu，Sai;Liu，Dongjuan;Zhong，Ming
成釉细胞瘤(Ameloblastoma，AB)是一种常见的牙源性上皮性肿瘤。Wnt/β-catenin途径与AB的侵袭有关。方法应用miRNAs和mRNAs芯片技术检测微RNA(MiRNAs)和信使RNAs(MRNAs)的表达变化，并用实时定量聚合酶链式反应(RT-PCR)进行验证。用Transwell法检测miR-29a-3p对AB细胞迁移和侵袭的影响。利用miR系统进行生物信息学预测，并通过定量RT-PCR、Western印迹和荧光素酶报告基因分析进行验证。结果在AB组织中过表达R-29a-3p，促进AB细胞的体外迁移和侵袭。连环蛋白β相互作用蛋白1(CTNNBIP1)是Wnt/β-连环蛋白途径的负调控因子，被认为是miR-29a-3p的靶标。结论miR-29a-3p通过靶向CTNNBIP1，通过WnT/β-catenin信号通路促进AB细胞的迁移和侵袭，抑制CTNNBIP1的表达，并促进其下游分子的表达。
BackgroundAmeloblastoma (AB) is a common epithelial odontogenic tumor. The Wnt/β‐catenin pathway has been found to be related to AB invasion.MethodsThe alteration expression of microRNAs (miRNAs) and messenger RNAs (mRNAs) was performed by miRNA and mRNA microarray analysis and validated by quantitative real‐time polymerase chain reaction (RT‐PCR). The effects of miR‐29a‐3p on migration and invasion in AB cells were evaluated by a transwell assay. Bioinformatic prediction was conducted using the miRSystem and validated by quantitative RT‐PCR, western blot, and a luciferase reporter assay.ResultsmiR‐29a‐3p was overexpressed in AB tissues, which promoted the migration and invasion of AB cells in vitro. Catenin beta interacting protein 1 (CTNNBIP1), a negative regulator of the Wnt/β‐catenin pathway, was predicted to be a target of miR‐29a‐3p. miR‐29a‐3p inhibited the expression of CTNNBIP1 and promoted the expression of the downstream molecules of the Wnt/β‐catenin pathway.ConclusionsmiR‐29a‐3p promoted migration and invasion in AB via Wnt/β‐catenin signaling by targeting CTNNBIP1.