Emergence and genetic variation of neuraminidase stalk deletions in avian influenza viruses.
Emergence and genetic variation of neuraminidase stalk deletions in avian influenza viruses.
复制标题
鸟类流感病毒中神经氨酸酶茎缺失的出现和遗传变异。
DOI：
10.1371/journal.pone.0014722
复制
发表时间：
2011-02-23
期刊：
影响因子：
3.7
通讯作者：
Carpenter TE
中科院分区：
文献类型：
--
作者：
Li J;Zu Dohna H;Cardona CJ;Miller J;Carpenter TE
当禽流感病毒(AIV)从其水库宿主(野生水禽和滨鸟)传播到家禽物种时，它们经历了与更高的毒力和更广泛的宿主范围有关的基因变化。家禽分离株病毒蛋白中常见的基因修饰是神经氨酸酶(NA)茎区域的缺失和血凝素(HA)上的糖基化位点的增加。尽管这些NA缺失突变发生在几个AIV亚型中，但它们还没有得到全面的分析。本研究分析了4,920个NA核苷酸序列、5,596个HA核苷酸序列和4,702个HA氨基酸序列，以阐明禽类寄主中广泛存在的NA柄缺失、NA柄缺失模式的遗传多态性以及NA柄缺失与HA氨基酸变异之间的关系。在N1-N3和N5-N7六个NA亚型中鉴定出47种不同的NA柄缺失模式。一项因共同祖先而控制了系统发育相关性的分析表明，NA茎的缺失与鸡类宿主和HA蛋白上的某些氨基酸特征在统计上相关。这些HA特征包括5个糖基化位点、1个插入和1个缺失。NA柄缺失与HA特征之间的相关性是HA-NA亚型特有的。我们的结果表明，在禽流感病毒NA蛋白中茎的缺失是相对常见的。了解NA柄缺失和相关的HA特征对于疫苗和药物的开发可能很重要，并可能有助于为家禽业建立有效的早期检测和预警系统。
When avian influenza viruses (AIVs) are transmitted from their reservoir hosts (wild waterfowl and shorebirds) to domestic bird species, they undergo genetic changes that have been linked to higher virulence and broader host range. Common genetic AIV modifications in viral proteins of poultry isolates are deletions in the stalk region of the neuraminidase (NA) and additions of glycosylation sites on the hemagglutinin (HA). Even though these NA deletion mutations occur in several AIV subtypes, they have not been analyzed comprehensively. In this study, 4,920 NA nucleotide sequences, 5,596 HA nucleotide and 4,702 HA amino acid sequences were analyzed to elucidate the widespread emergence of NA stalk deletions in gallinaceous hosts, the genetic polymorphism of the deletion patterns and association between the stalk deletions in NA and amino acid variants in HA. Forty-seven different NA stalk deletion patterns were identified in six NA subtypes, N1–N3 and N5–N7. An analysis that controlled for phylogenetic dependence due to shared ancestry showed that NA stalk deletions are statistically correlated with gallinaceous hosts and certain amino acid features on the HA protein. Those HA features included five glycosylation sites, one insertion and one deletion. The correlations between NA stalk deletions and HA features are HA-NA-subtype-specific. Our results demonstrate that stalk deletions in the NA proteins of AIV are relatively common. Understanding the NA stalk deletion and related HA features may be important for vaccine and drug development and could be useful in establishing effective early detection and warning systems for the poultry industry.
登录
查看更多内容
影响因子：
3.7
作者：
ELS, MC;AIR, GM;LAVER, WG
通讯作者：
LAVER, WG
影响因子：
11.8
作者：
Hirst M;Astell CR;Griffith M;Coughlin SM;Moksa M;Zeng T;Smailus DE;Holt RA;Jones S;Marra MA;Petric M;Krajden M;Lawrence D;Mak A;Chow R;Skowronski DM;Tweed SA;Goh S;Brunham RC;Robinson J;Bowes V;Sojonky K;Byrne SK;Li Y;Kobasa D;Booth T;Paetzel M
通讯作者：
Paetzel M
影响因子：
1.4
作者：
Dunn, PA;Wallner-Pendleton, EA;Davison, S
通讯作者：
Davison, S
影响因子：
2.8
作者：
Capua, I;Mutinelli, F;Alexander, DJ
通讯作者：
Alexander, DJ
影响因子：
5.4
作者：
Cheung, C. L.;Vijaykrishna, D.;Guan, Y.
通讯作者：
Guan, Y.