The Jumonji gene family in Crassostrea gigas suggests evolutionary conservation of Jmj-C histone demethylases orthologues in the oyster gametogenesis and development
The Jumonji gene family in Crassostrea gigas suggests evolutionary conservation of Jmj-C histone demethylases orthologues in the oyster gametogenesis and development
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DOI：
10.1016/j.gene.2013.12.016
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发表时间：
2014-03-15
期刊：
影响因子：
3.5
通讯作者：
Riviere, Guillaume
中科院分区：
文献类型：
--
作者：
Fellous, Alexandre;Favrel, Pascal;Riviere, Guillaume
Jumonji（Jmj）蛋白是组蛋白脱甲基酶，其控制干细胞的身份。从植物到哺乳动物，Jmj基因的特征都与发育的表观遗传调控有关。尽管太平洋牡蛎Crassostrea gigas代表了世界上最重要的水产养殖资源之一，但对这种Lophotrochozoan物种胚胎发生和繁殖的分子机制仍然知之甚少。然而，C. gigas EST文库的结果表明，存在推定的Jumonji基因，提出了这个家庭的组蛋白去甲基化酶在牡蛎的保护问题。使用引物步移，5 '-RACE PCR和计算机分析，我们在牡蛎，称为Cg-Jmj，轴承保守结构域的推定组蛋白脱甲基酶活性的关键Jumonji直向同源物的特征。系统发育分析表明，牡蛎Jumonji聚类成两个不同的组：“单域Jmj”和“多域Jmj”，并定义了8个亚组对应的每一个同源直系同源后生动物。RT-qPCR研究表明Cg-Jmj mRNA在早期发育和沿着生殖周期中具有特异性调控。此外，在原位和在toto杂交表明，牡蛎Jumonji基因主要是在成年牡蛎的性腺内转录，而它们在胚胎和幼虫发育过程中显示出无处不在的表达。我们的研究表明，9 Jumonji直向同源的牡蛎C gigas的存在。它们的结构域保守性和它们的表达谱暗示了在生殖和发育过程中的意义，质疑了由组蛋白甲基化在网状毛管动物中的表观遗传调节。(C)2013爱思唯尔有限公司版权所有。
Jumonji (Jmj) proteins are histone demethylases, which control the identity of stem cells. Jmj genes were characterized from plants to mammals where they have been implicated in the epigenetic regulation of development. Despite the Pacific oyster Crassostrea gigas representing one of the most important aquaculture resources worldwide, the molecular mechanisms governing the embryogenesis and reproduction of this lophotrochozoan species remain poorly understood. However, annotations in the C. gigas EST library suggested the presence of putative Jumonji genes, raising the question of the conservation of this family of histone demethylases in the oyster. Using Primer walking, 5'-RACE PCR and in silico analyses, we characterized nine Jumonji orthologues in the oyster, called Cg-Jmj, bearing conserved domains critical for putative histone demethylase activity. Phylogenic analyses revealed that oyster Jumonji cluster into two distinct groups: 'single-domain Jmj' and 'multi-domain Jmj', and define 8 subgroups corresponding to each cognate orthologues in metazoans. RT-qPCR investigations showed specific regulations of Cg-Jmj mRNAs during the early development and along the reproduction cycle. Furthermore, in situ and in toto hybridizations indicate that oyster Jumonji genes are transcribed mostly within the gonad in adult oysters whereas they display a ubiquitous expression during embryonic and larval development. Our study demonstrates the presence of nine Jumonji orthologues in the oyster C gigas. Their domain conservation and their expression profile suggest an implication during reproduction and development, questioning about the epigenetic regulation by histone methylation in lophotrochozoans. (C) 2013 Elsevier B.V. All rights reserved.